Bacteroides fragilis 장독소 자극에 의한 인체 장상피세포에서의 Cyclooxygenase-2 발현

• Published in: Journal of bacteriology and virology Vol. 32; no. 2; pp. 147 - 157
• Main Authors: 김정목, Jung Mogg Kim, 강신재, Shin Jae Kang, 조수진, Soo Jin Cho, 정훈용, Hwoon Yong Jung, 오유경, Yu Kyoung Oh, 김영전, Young Jeon Kim
• Format: Journal Article
• Language: Korean
• Published: 대한바이러스학회 2002; 대한미생물학회
• Subjects: Bacteroides fragilis enterotoxin; Cyclooxygenase-2; NF-kB; Prostaglandin; 생물학; Cyclooxygenase-2; Prostaglandin; Bacteroides fragilis enterotoxin; NF- ${\kappa}B
• ISSN: 1598-2467; 2093-0429

A ${\sim}20\;kDa$ heat-labile toxin (EFT) produced by enterotoxigenic B. fragilis induces chemokine responses that may be associated with mucosal inflammation. To determine whether epithelial cells can contribute to BFT-induced inflammation, we assessed the expression of cyclooxygenase (COX)-2 in BFT-stimulated human intestinal epithelial cells. Human intestinal epithelial cell lines, HT-29 and Caco-2, were incubated with purified BFT. COX-2 mRNA and protein expression were measured by quantitative RT-PCR and Western blot analysis, respectively. BFT increased expression of COX-2, not that of COX-1, in human intestinal epithelial cell lines. Up-regulated COX-2 expression was paralleled by increased prostaglandin $E_2\;(PGE_2)$ production measured by the radioimmunoassay. $PGE_2$ was predominantly secreted from the basolateral surface of BFT-treated epithelial cells, whereas lactate dehydrogenase was released predominantly from the apical surface. Moreover, the COX-2 expression and $PGE_2$ production were significantly suppressed when NF-${\kappa}B$ activity was inhibited. The basolateral secretion of $PGE_2$ by up-regulated COX-2 in the BFT-stimulated colon epithelial cells seems to contribute to the inflammatory response in the underlying intestinal mucosa.