ARTIFICIAL BLOOD 〜 POSSIBILITY OF FUTURE PREHOSPITAL TRAUMA RESUSCITATION

• Published in: Journal of the Japanese Association for the Surgery of Trauma Vol. 35; no. 4; pp. 275 - 282
• Main Authors: SAITOH, Daizoh, KINOSHITA, Manabu, SAKAI, Hiromi, TAKEOKA, Shinji, ISHIDA, Osamu, HAGISAWA, Kohsuke
• Format: Journal Article
• Language: Japanese
• Published: The Japanese Association for the Surgery of Trauma 2021; 一般社団法人 日本外傷学会
• Subjects: acute thrombocytopenic rabbit model; Hemorrhagic shock; platelet substitutes; prehospital resuscitation; red blood cell substitutes; 人工血小板; 人工赤血球; 出血性ショック; 家兎急性血小板減少モデル; 病院前蘇生
• ISSN: 1340-6264; 2188-0190
• DOI: 10.11382/jjast.35.4_05

We developed an artificial blood that has potent hemostatic and oxygen carrying effects, and requires neither blood typing nor crossmatch testing. Our artificial blood consisting of platelet substitutes [H12- (ADP) -liposomes] and red blood cell substitutes [hemoglobin vesicles, a cellular-type hemoglobin-based oxygen carrier] can be stored for a long period at room temperature without shaking. H12- (ADP) -liposomes bear synthetic HHLGGAKQAGDV (H12) peptides corresponding to the carboxyl terminal of the fibrinogen γ-chain on their surface and encapsulate the physiological platelet agonist adenosine diphosphate (ADP). They can accumulate at bleeding sites and promote platelet thrombi even under thrombocytopenic coagulopathy. In acute thrombocytopenic rabbits with noncompressible penetrating liver injury, combination therapy with intravenous infusions of H12- (ADP) -liposomes followed by hemoglobin vesicles enabled the rescue from severe massive hemorrhage with coagulopathy due to the potent hemostatic and oxygen carrying effects. Our artificial blood may be useful for damage control resuscitation of trauma-induced massive hemorrhage, especially pre-hospital resuscitation.