Fundamental study of “Lumipulse Presto IL-2R” reagent for measuring soluble interleukin-2 receptor with Lumipulse L2400

• Published in: Japanese Journal of Medical Technology Vol. 69; no. 2; pp. 209 - 214
• Main Authors: FUKUDA, Tetsuya, NAKADA, Yumeto, ICHIKAWA, Hitomi, MOTOKURA, Toru, NOGAMI, Satoshi, WATANABE, Kanako
• Format: Journal Article
• Language: Japanese
• Published: Japanese Association of Medical Technologists 01.04.2020; 一般社団法人 日本臨床衛生検査技師会
• Subjects: CLEIA; Lumipulse Presto IL-2R; serum sample; soluble interleukin-2 receptor; STACIA CLEIA IL-2R; ステイシアCLEIA IL-2R; ルミパルスプレストIL-2R; 化学発光酵素免疫測定法; 可溶性インターロイキン-2受容体; 血清検体
• ISSN: 0915-8669; 2188-5346
• DOI: 10.14932/jamt.19-104

The levels of the soluble interleukin-2 receptor in serum are elevated in non-Hodgkin’s lymphoma and adult T-cell leukemia/lymphoma and reflect disease activity, so they are used for the assessment of treatment response, auxiliary diagnosis, and follow-up. Since the reagent “Lumipulse Presto IL-2R” that can be used with Lumipulse L2400 was developed this time, a basic study was carried out to introduce the reagent. Good results were obtained for within-run precision, between-day precision, dilution linearity, detection limit, and quantification limit. No effects of coexisting substances or container types were found. The correlation coefficient with “STACIA CLEIA IL-2R” was as high as 0.995, the regression equation was y = 1.17x − 34.33, and no extremely deviated sample was found. In conclusion, because of its good performance, it was suggested that the reagent can be introduced.