H1.0 induces paclitaxel‐resistance genes expression in ovarian cancer cells by recruiting GCN5 and androgen receptor

• Published in: Cancer science Vol. 113; no. 8; pp. 2616 - 2626
• Main Authors: Kohli, Abhidha, Huang, Shang‐Lang, Chang, Ting‐Chang, Chao, Chuck C.‐K., Sun, Nian‐Kang
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.08.2022; John Wiley and Sons Inc
• Subjects: 1-Phosphatidylinositol 3-kinase; Acetylation; AKT protein; androgen receptor; Androgen receptors; Androgens; Binding sites; Cell cycle; Chemoresistance; Ectopic expression; Epigenetics; GCN5; Gene expression; Genetic engineering; Genomics; H1.0; Histone H1; Kinases; Medical prognosis; mRNA; Original; Ovarian cancer; Paclitaxel; paclitaxel resistance; Plasmids; Proteins; Signal transduction; Survival analysis; GCN5; H1.0; paclitaxel resistance; androgen receptor; ovarian cancer
• ISSN: 1347-9032; 1349-7006; 1349-7006
• DOI: 10.1111/cas.15448

More than 90% of ovarian cancer deaths are due to relapse following development of chemoresistance. Our main objective is to better understand the molecular mechanism underlying paclitaxel resistance (taxol resistance, Txr) in ovarian cancer. Here, we observed that the linker histone H1.0 is upregulated in paclitaxel‐resistant ovarian cancer cells. Knockdown of H1.0 significantly downregulates the androgen receptor (AR) and sensitizes paclitaxel‐resistant SKOV3/Txr and 2774/Txr cell lines to paclitaxel. Conversely, ectopic expression of H1.0 upregulates AR and increases Txr in parental SKOV3 and MDAH2774 cells. Notably, H1.0 upregulation is associated with disease recurrence and poor survival in a subset of ovarian cancer subjects. Inhibition of PI3K significantly reduces H1.0 mRNA and protein levels in paclitaxel‐resistant cells, suggesting the involvement of the PI3K/AKT signaling pathway. Knockdown of H1.0 and AR also downregulates the Txr genes ABCB1 and ABCG2 in paclitaxel‐resistant cells. Our data show that H1.0 induces GCN5 expression and histone acetylation, thereby enhancing Txr gene transactivation. These findings suggest that Txr in ovarian cancer involves the PI3K/AKT pathway and leads to upregulation of histone H1.0, recruitment of GCN5 and AR, followed by upregulation of a subgroup of Txr genes that include ABCB1 and ABCG2. This study is the first report describing the relationship between histone H1.0 and GCN5 that cooperate to induce AR‐dependent Txr in ovarian cancer cells. To better understand the molecular mechanism underlying taxol resistance (Txr), we explored the axis PI3K/AKT/H1.0 recruiting GCN5 and AR to upregulate Txr genes, ABCB1 and ABCG2. This study is the first report describing the relationship between histone H1.0 and GCN5 which cooperate to induce AR‐dependent Txr in ovarian cancer cells.