口腔がん組織由来の赤色蛍光：がん検知の可能性

• Published in: Transactions of Japanese Society for Medical and Biological Engineering Vol. 54Annual; no. 26PM-Abstract; p. S89
• Main Authors: 小島, 涼, 増本, 一真, 鬼木, 玲奈, 寺原, 航, 仲谷, 将隆, 守本, 祐司, 長井, 健一郎, 江南, 慧, 小倉, 俊一郎, 長田, 哲次, 四ノ宮, 成祥
• Format: Journal Article
• Language: Japanese
• Published: 公益社団法人 日本生体医工学会 2016; Japanese Society for Medical and Biological Engineering
• ISSN: 1347-443X; 1881-4379
• DOI: 10.11239/jsmbe.54Annual.S89

IntroductionWhen oral cancer tissue is irradiated with blue light, red fluorescence is sometimes seen. We think the fluorescence can be a new biomarker for cancer proliferation. Hence, in this study we investigated the properties of the red fluorescence and also examined whether the fluorescence was seen in the proliferative oral tissue other than cancer.Materials & MethodsEighteen oral proliferative samples were used in this study. Nine of them were gingival cancer. The most of tissue type was squamous cell carcinoma (11 of the 18 specimens). Fluorescence image of the samples was obtained using an in-vivo imaging system (IVIS, ParkinElmer) with an excitation filter of 420 nm and an emission filter of 620nm.Results & DiscussionThe red fluorescence was seen in 7 of the 18 samples (39%). The fluorescence was not homogeneously distributed in each samples, rather spotty, patchy distribution. When the fluorescent specimens were cut into small pieces, the fluorescence was observed even from the cut surface, suggesting that the fluorescence originates from substances inside cancer tissue. On the other hand, the fluorescence was also seen in granulation tissue (1 of the 3 specimen), thus the fluorescence would not arise from the materials that are unique to cancer cells.ConclusionThe red fluorescence was seen in approximately 40% of oral proliferative tissue. We intend to identify the origin of the fluorescence and examine whether it can be used for cancer detection.