膜電位/Ca2+同時光学マッピングを用いたCa2+バーストの解析

• Published in: Transactions of Japanese Society for Medical and Biological Engineering Vol. 54Annual; no. 26AM-Abstract; p. S61
• Main Authors: 野口, 展士, 立柳, 紀林, 荒船, 龍彦, 柴田, 仁太郎, 本荘, 晴朗, 本間, 章彦, 山崎, 正俊
• Format: Journal Article
• Language: Japanese
• Published: 公益社団法人 日本生体医工学会 2016; Japanese Society for Medical and Biological Engineering
• ISSN: 1347-443X; 1881-4379
• DOI: 10.11239/jsmbe.54Annual.S61

Spiral reentry is thought that the abnormal excitation propagation during Ventricular tachycardia (VT) and fibrillation (VF). It was reported that intracellular calcium (Cai) rise just before VT develop VF which phanomenon is called "Cai burst". However interaction between Cai burst and VF initiation were still unclear. In this study, analyze between spatial heterogeneous Action Potential Duration (APD) and Cai amplitude during VT. Simultaneous optical mapping Vm/Cai was performed with Langendorff-perfused rabbit hearts during pacing and VF induced cross-field stimulation protocol. APD and Cai amplitude maps were constructed during pacing and VT both. APD and Cai amplitude altered spatially which magnitude increase each beat in VT. Cai amplitude alternans were larger than APD alternans. This results spatially discordant APD alternans and reentry generation. This results suggested that functional block line emerged alternans in VT is mechanisms of reentry generation.