In vitro antibacterial activity of temafloxacin

• Published in: CHEMOTHERAPY Vol. 41; no. Supplement5; pp. 67 - 80
• Main Authors: Onta, Tokio, Oshida, Tadahiro, Naito, Machiko, Yamaguchi, Totaro, Matsushita, Tadahiro
• Format: Journal Article
• Language: English; Japanese
• Published: Japanese Society of Chemotherapy 1993; 公益社団法人 日本化学療法学会
• Subjects: DNA gyrase; 形態変化; 抗菌力
• ISSN: 0009-3165; 1884-5894
• DOI: 10.11250/chemotherapy1953.41.Supplement5_67

The antibacterial activity of a new quinolone, temafloxacin (TMFX), was compared with that of ciprofloxacin (CPFX), enoxacin (ENX), norfloxacin (NFLX) and ofloxacin (OFLX), using clinical isolates. The activity of TMFX against gram-positive bacteria was superior to that of the reference quinolones. The activity of CPFX was superior to that of TMFX against many kinds of gram-negative bacteria, but against Haemophilus influenzae and Moraxella catarrhalis, TMFX was equal to CPFX. Against Bacteroides fragilis, TMFX was superior to the reference quinolones. The antibacterial activity of TMFX was influenced by the pH of the medium and the addition of Mg2+ into the medium, as was that of the reference quinolones. TMFX acted bactericidally on Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa at higher concentrations than MIC or 2MIC for the respective bacteria. Microscopic observation of E. coli and P. aeruginosa treated with TMFX and OFLX showed that both drugs changed E. coli cells to filamentous form, and some P. aeruginosa cells to a bulging form at higher concentrations than 8MIC. The IC50 (50% inhibitory concentration) of TMFX for DNA gyrase of E. coli KL-16 was the same as that of OFLX. The frequency of isolation of strains resistant to TMFX at 8MIC was the same as that of the reference quinolones. It was suggested that TMFX would be effective against many bacterial infections.