Analysis of the Fraction of Individual Bile Acids in Human Bile Using High-performance Liquid Chromatography

• Published in: Japanese Journal of National Medical Services Vol. 42; no. 3; pp. 216 - 219
• Main Authors: TAKAMATSU, Keiju, KUDO, Kyoko, TAKADA, Yoshimitsu, WARIISHI, Koichi, ISHIYAMA, Kazuo
• Format: Journal Article
• Language: Japanese
• Published: Japanese Society of National Medical Services 1988; 一般社団法人 国立医療学会
• Subjects: BOND ELUT C18; 胆汁中胆汁酸; 高速液体クロマトグラフィー
• ISSN: 0021-1699; 1884-8729
• DOI: 10.11261/iryo1946.42.216

We tried to determine the fraction of individual bile acids in human serum in order to have some informations which could not be obtained by the determination of total bile acid in human serum, and already reported about it. Furthermore, we thought it necessary to get more detailed clinical profile by elucidating the composing mechanism of the bile acids in liver, and we measured individual bile acids in human bile using high-performance liquid chromatography. Since the concentration of bile acid in human bile is much higher than in human serum, and the concentration of conjugated bile acids is over one hundred times as high as that of free bile acids, it is hard to extract both of them simultaneously from human bile by BOND ELUT C18 because of a big difference. Therefore, we diluted them appropriately with normal saline and determined them separately. As a result, we could obtain the high recovery rate almost equal to that of the extraction of bile acids from human serum.