PURIFICATION OF T LYMPHOCYTES FROM HUMAN PERIPHERAL BLOOD BY THE ADSORBENT WITH POLYSACCHARIDE-LIGAND

It was found that the adsorbent containing a polysaccharide had a selectivity for B lymphocytes, granulocytes and monocytes. Especially, the adsorbent with pectin (EC-PEC) represented the excellent property for purifying of T lymphocytes. Therefore, pectin as an affinity ligand was available for dir...

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Bibliographic Details
Published inJinko Zoki Vol. 18; no. 3; pp. 1397 - 1400
Main Authors YURA, H., TAKAHASHI, A., AKAIKE, T., SAMESHIMA, T., YAMAMOTO, Y., NAGOYA, M.
Format Journal Article
LanguageJapanese
Published JAPANESE SOCIETY FOR ARTIFICIAL ORGANS 1989
一般社団法人 日本人工臓器学会
Subjects
adsorbent
B lymphocytes
human peripheral blood
polysaccharide
purification
T
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ISSN0300-0818
1883-6097
DOI10.11392/jsao1972.18.1397

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Summary:It was found that the adsorbent containing a polysaccharide had a selectivity for B lymphocytes, granulocytes and monocytes. Especially, the adsorbent with pectin (EC-PEC) represented the excellent property for purifying of T lymphocytes. Therefore, pectin as an affinity ligand was available for direct enrichment of T lymphocytes from human peripheral blood. It seemed that selectivity of EC-PEC to white blood cells was based on characteristics of the carboxyl groups and the flexibility of polysaccharide chain.
ISSN:0300-0818
1883-6097
DOI:10.11392/jsao1972.18.1397