A Novel Method for Measuring Serum C-Reactive Protein by Multilayer Dry Chemistry

• Published in: Japanese Journal of Clinical Chemistry Vol. 23; no. 4; pp. 305 - 309
• Main Authors: Shinoki, Hiroshi, Ogawa, Masashi, Sugihara, Mitsuru, Tanimoto, Tetsuji, Makino, Yoshihiko, Nishizono, Isao, Ashihara, Yoshihiro, Hiraoka, Toshikage, Hora, Naohumi, Ninomiya, Tadashi, Kasahara, Yasushi
• Format: Journal Article
• Language: Japanese
• Published: Japan Society of Clinical Chemistry 1994; 一般社団法人 日本臨床化学会
• Subjects: amylase-conjugated antibody; CRP; dry chemistry; homogeneous EIA; multilayer dry film; urgent-stat analysis; アミラーゼ標識抗体; ドライケミストリー法; 即時・緊急検査; 均一系酵素免疫反応; 多層分析フィルム
• ISSN: 0370-5633; 2187-4077
• DOI: 10.14921/jscc1971b.23.4_305

Dry chemistry technology is well established in the clinical chemistry laboratory because of its simplicity and rapidity. Without preparation of reagents, the concentrations of blood components can easily be determined. CRP is measured for diagnosis of early inflammation and tissue injury. We have developed a multilayer dry film for quantification of serum CRP which is measured on a Fuji DRI-CHEM Analyzer. The principle involved is that of homogeneous enzyme immunoassay. The hydrolyzing activity for insoluble starch of amylase conjugated to an antibody is modulated by binding of macromolecular antigen (CRP) to the conjugate. The film has three major layers: a reaction layer for immunological and enzymatic reactions, a barrier layer, and a color formation layer. Precision and comparison studies gave good results. The lower detection limit was approximately 0.5mg/100ml.