Measurement of Lipid Peroxides in Apo B-Containing Lipoprotein Fraction in Plasma

• Published in: Japanese Journal of Clinical Chemistry Vol. 25; no. 3; pp. 157 - 164
• Main Authors: Shishino, Koji, Murase, Mitsuharu, Saheki, Shuichi, Takeuchi, Nozomu, Tokunaga, Kenji
• Format: Journal Article
• Language: Japanese
• Published: Japan Society of Clinical Chemistry 1996; 一般社団法人 日本臨床化学会
• Subjects: apolipoprotein B; diabetes mellitus; lipid peroxide; oxidized LDL; TBA reactants; TBA反応性物質; アポリポプロテインB; 糖尿病; 過酸化脂質; 酸化LDL
• ISSN: 0370-5633; 2187-4077
• DOI: 10.14921/jscc1971b.25.3_157

Measurement of lipid peroxides (LPO) in apo B-containing lipoproteins is reported. LPO was measured by fluorometry after thiobarbituric acid reaction in immunoprecipitates with antiapo B serum. Inter- and intra-assay precisions were below 6.0% and 7.0% CV, respectively and the normal reference range was 0.30-1.52nmol/ml (±2SD). Correlation between the proposed method and the current phosphotungstic acid/magnesium chloride method was good; (r=0.931, y=0.864x-0.05). The distribution of plasma LPO was 21.8% to the apo B-containing fraction and 78.4% to the non-apo B fraction. Diabetes mellitus patients with vascular complications had significantly higher values and those without complications had slightly higher LPO levels in plasma and LDL or HDL fraction compared to those normal subjects