Enhancement of chromatographic performances of porous polymer packings by diminishing their particle diameter

• Published in: BUNSEKI KAGAKU Vol. 36; no. 9; pp. 557 - 560
• Main Authors: MIYAGI, Hiroyuki, MIZUNO, Masako, KAMEZAWA, Norimasa, MIURA, Junkichi
• Format: Journal Article
• Language: Japanese
• Published: The Japan Society for Analytical Chemistry 05.09.1987
• Subjects: correlation between particle diameter and height equivalent to a theoretical plate; porous polymer packings for HPLC; quaternarized styrene-divinylbenzene co-polymer
• ISSN: 0525-1931
• DOI: 10.2116/bunsekikagaku.36.9_557

Correlation between chromatographic performances and the physical properties of porous polymer packings, such as particle diameter, specific surface area, pore diameter and pore volume was studied. The porous polymer packings were prepared by quaternarizing styrene-divinylbenzene co-polymer. The chromatographic performances depended mainly on the particle diameter of the polymer packings. By decreasing the particle diameter (dp), theoretical plates (H) and pressure resistibility (P) of the packings were enhanced. The column packed with 2.5 μm packings showed 1 × 105 plates/m of H for uracil and 200 kg/cm2 of P, which were twice of H and 30% increase in P as compared with a commercially available polymer packings. Four nucleic acid base mixture, consisting of cytosine, uracil, guanine and adenine, was separated within 2 min on this column, which was 3-fold reduction of separation time measured with the commercial packings.