cyp51A遺伝子バリアントによる日本産Aspergillus oryzaeのA. flavusからの判別の試み
Aspergillus section Flaviに属する菌種には, 日本の伝統的醸造に重要なAspergillus oryzaeと, アフラトキシン産生能を有するA. flavusが含まれる. 両菌種を遺伝学的に判別するのは, ゲノムが99%以上同一のため非常に至難である. 近年, 両菌種の判別指標としてcyp51A遺伝子バリアントが提唱された. 今回, 本指標を日本産A. oryzaeに適用したところ, 判別結果が菌種に合致しない菌株が複数, 見出された....
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| Published in | マイコトキシン p. 75-2-1 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English Japanese |
| Published |
日本マイコトキシン学会
31.07.2025
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0285-1466 1881-0128 |
| DOI | 10.2520/myco.75-2-1 |
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| Abstract | Aspergillus section Flaviに属する菌種には, 日本の伝統的醸造に重要なAspergillus oryzaeと, アフラトキシン産生能を有するA. flavusが含まれる. 両菌種を遺伝学的に判別するのは, ゲノムが99%以上同一のため非常に至難である. 近年, 両菌種の判別指標としてcyp51A遺伝子バリアントが提唱された. 今回, 本指標を日本産A. oryzaeに適用したところ, 判別結果が菌種に合致しない菌株が複数, 見出された. |
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| AbstractList | Aspergillus section Flaviに属する菌種には, 日本の伝統的醸造に重要なAspergillus oryzaeと, アフラトキシン産生能を有するA. flavusが含まれる. 両菌種を遺伝学的に判別するのは, ゲノムが99%以上同一のため非常に至難である. 近年, 両菌種の判別指標としてcyp51A遺伝子バリアントが提唱された. 今回, 本指標を日本産A. oryzaeに適用したところ, 判別結果が菌種に合致しない菌株が複数, 見出された. |
| Author | 山上, 陽平 金刺, 佑平 矢部, 希見子 古川, 智宏 久城, 真代 |
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| References | 2) Varga, J.; Frisvad, J.C.; Samson, R.A. Two new aflatoxin producing species and an overview of Aspergillus section Flavi. Stud. Mycol. 2011, 69, 57-80. 3) NRIB The Story of Sake. https://www.nrib.go.jp/English/sake/pdf/SakeNo02_en.pdf. Accessed on 27th Feb., 2025. 1) Frisvad, J.C.; Hubka, V.; Ezekiel, C.; Hong, S.-B.; Nováková, A.; Chen, A.; Arzanlou, M.; Larsen, T.; Sklenář, F.; Mahakarnchanakul, W.; Samson, R.A.; Houbraken, J. Taxonomy of Aspergillus section Flavi and their production of aflatoxins, ochratoxins and other mycotoxins. Stud. Mycol. 2019, 93, 1-63. 11) Rank, C.; Klejnstrup, M.L.; Petersen, L.M.; Kildgaard, S.; Frisvad, J.C.; Held Gotfredsen, C.; Ostenfeld Larsen, T. Comparative Chemistry of Aspergillus oryzae (RIB40) and A. flavus (NRRL 3357). Metabolites 2012, 2, 39-56. 8) Hedayati, M.T.; Taghizadeh-Armaki, M.; Zarrinfar, H.; Hoseinnejad, A.; Ansari, S.; Abastabar, M.; Er, H.; Özhak, B.; Öğünç, D.; Ilkit, M.; Seyedmousavi, S. Discrimination of Aspergillus flavus from Aspergillus oryzae by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry. Mycoses 2019, 62, 1182-1188. 9) Nargesi, S.; Abastabar, M.; Valadan, R.; Mayahi, S.; Youn, J.-H.; Hedayati, M.T.; Seyedmousavi, S. Differentiation of Aspergillus flavus from Aspergillus oryzae targeting the cyp51A gene. Pathogens 2021, 10, 1279. 5) Tanaka, K.; Goto, T.; Manabe, M.; Matsuura, S. Traditional Japanese fermented foods free from mycotoxin contamination. Jpn. Agric. Res. Quart. 2002, 36, 45-50. 7) Masih, A.; Singh, P.K.; Kathuria, S.; Agarwal, K.; Meis, J.F.; Chowdhary, A. Identification by molecular methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry and antifungal susceptibility profiles of clinically significant rare Aspergillus species in a referral chest hospital in Delhi, India. J. Clin. Microbiol. 2016, 54, 2354-2364. 4) Barbesgaard, P.; Heldt-Hansen, H.P.; Diderichsen, B. On the safety of Aspergillus oryzae: A review. Appl. Microbiol. Biotechnol. 1992, 36, 569-572. 6) Rokas, A.; Payne, G.; Fedorova, N.D.; Baker, S.E.; Machida, M.; Yu, J.; Georgianna, D.R.; Dean, R.A.; Bhatnagar, D.; Cleveland, T.E.; Wortman, J.R.; Maiti, R.; Joardar, V.; Amedeo, P.; Denning, D.W.; Nierman, W.C. What can comparative genomics tell us about species concepts in the genus Aspergillus? Stud. Mycol. 2007, 59, 11-17. 10) Pitt, J.I.; Hocking, A.D.; Glenn, D.R. An improved medium for the detection of Aspergillus flavus and A. parasiticus. J. Appl. Bacteriol. 1983, 54, 109-114. |
| References_xml | – reference: 11) Rank, C.; Klejnstrup, M.L.; Petersen, L.M.; Kildgaard, S.; Frisvad, J.C.; Held Gotfredsen, C.; Ostenfeld Larsen, T. Comparative Chemistry of Aspergillus oryzae (RIB40) and A. flavus (NRRL 3357). Metabolites 2012, 2, 39-56. – reference: 7) Masih, A.; Singh, P.K.; Kathuria, S.; Agarwal, K.; Meis, J.F.; Chowdhary, A. Identification by molecular methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry and antifungal susceptibility profiles of clinically significant rare Aspergillus species in a referral chest hospital in Delhi, India. J. Clin. Microbiol. 2016, 54, 2354-2364. – reference: 6) Rokas, A.; Payne, G.; Fedorova, N.D.; Baker, S.E.; Machida, M.; Yu, J.; Georgianna, D.R.; Dean, R.A.; Bhatnagar, D.; Cleveland, T.E.; Wortman, J.R.; Maiti, R.; Joardar, V.; Amedeo, P.; Denning, D.W.; Nierman, W.C. What can comparative genomics tell us about species concepts in the genus Aspergillus? Stud. Mycol. 2007, 59, 11-17. – reference: 1) Frisvad, J.C.; Hubka, V.; Ezekiel, C.; Hong, S.-B.; Nováková, A.; Chen, A.; Arzanlou, M.; Larsen, T.; Sklenář, F.; Mahakarnchanakul, W.; Samson, R.A.; Houbraken, J. Taxonomy of Aspergillus section Flavi and their production of aflatoxins, ochratoxins and other mycotoxins. Stud. Mycol. 2019, 93, 1-63. – reference: 10) Pitt, J.I.; Hocking, A.D.; Glenn, D.R. An improved medium for the detection of Aspergillus flavus and A. parasiticus. J. Appl. Bacteriol. 1983, 54, 109-114. – reference: 3) NRIB The Story of Sake. https://www.nrib.go.jp/English/sake/pdf/SakeNo02_en.pdf. Accessed on 27th Feb., 2025. – reference: 9) Nargesi, S.; Abastabar, M.; Valadan, R.; Mayahi, S.; Youn, J.-H.; Hedayati, M.T.; Seyedmousavi, S. Differentiation of Aspergillus flavus from Aspergillus oryzae targeting the cyp51A gene. Pathogens 2021, 10, 1279. – reference: 2) Varga, J.; Frisvad, J.C.; Samson, R.A. Two new aflatoxin producing species and an overview of Aspergillus section Flavi. Stud. Mycol. 2011, 69, 57-80. – reference: 4) Barbesgaard, P.; Heldt-Hansen, H.P.; Diderichsen, B. On the safety of Aspergillus oryzae: A review. Appl. Microbiol. Biotechnol. 1992, 36, 569-572. – reference: 8) Hedayati, M.T.; Taghizadeh-Armaki, M.; Zarrinfar, H.; Hoseinnejad, A.; Ansari, S.; Abastabar, M.; Er, H.; Özhak, B.; Öğünç, D.; Ilkit, M.; Seyedmousavi, S. Discrimination of Aspergillus flavus from Aspergillus oryzae by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry. Mycoses 2019, 62, 1182-1188. – reference: 5) Tanaka, K.; Goto, T.; Manabe, M.; Matsuura, S. Traditional Japanese fermented foods free from mycotoxin contamination. Jpn. Agric. Res. Quart. 2002, 36, 45-50. |
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| SubjectTerms | Aspergillus flavus Aspergillus section Flavi アフラトキシン 種同定 |
| Title | cyp51A遺伝子バリアントによる日本産Aspergillus oryzaeのA. flavusからの判別の試み |
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