Polymorphic distribution and phenotypic diversity of Frankia strains in nodule lobes of Hippöphae salicifolia D. Don

Frankia, as a source of symbiotic N₂ fixation in nonleguminous actinorhizal plants has received tremendous importance in the last few decades. Studies pertaining to the determination of genetic diversity of Frankia strains in diversified groups of actinorhizal plants have been a subject of attention...

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Published inCurrent science (Bangalore) Vol. 90; no. 11; pp. 1516 - 1521
Main Authors Sarma, Hridip Kumar, Sharma, Bipin Kumar, Singh, Satya Shila, Tiwari, S. C., Mishra, Arun Kumar
Format Journal Article
LanguageEnglish
Published Current Science Association 10.06.2006
Subjects
Actinomycetes
Axenic culture
Carbon
Frankia
Liquids
Nitrogen
Plants
RESEARCH COMMUNICATIONS
Root nodules
Sporangia
Symbiosis
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ISSN0011-3891

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Summary:Frankia, as a source of symbiotic N₂ fixation in nonleguminous actinorhizal plants has received tremendous importance in the last few decades. Studies pertaining to the determination of genetic diversity of Frankia strains in diversified groups of actinorhizal plants have been a subject of attention in recent times. We report here an attempt to isolate and identify consortia of Frankia strains from root nodules of Hippöphae salicifolia D. Don collected from the eastern Himalayas of north Sikkim. A total 31 Frankia axenic cultures were isolated from 27 root nodule lobes which differed in colouration and morphological pattern. The isolates were subjected to different formulation of carbon sources and pH variations with and without additional exogenous N₂ source. Finally a total of 11 single spore isolates were identified based on morphological and physiological differences. The present study reveals that there is an existence of a great deal of heterogeneity among the Frankia strains associated with Hippöphae salicifolia D. Don.
ISSN:0011-3891