MD/MS AND OSTEOCALSIN METHOD FOR STUDY OF OSTEOPOROSIS

• Published in: Journal of The Showa Medical Association Vol. 50; no. 4; pp. 368 - 381
• Main Author: YOSHIDA, Minoru
• Format: Journal Article
• Language: English; Japanese
• Published: The Showa University Society 1990; 昭和大学学士会
• Subjects: csteoporosis; Gla; HPLC; MD/MS method; MD/MS法; osteocalcin; γ-カルボキシグルタミン酸; オステオカルシン; 骨粗鬆症; 高速液体クグラフィー
• ISSN: 0037-4342; 2185-0976
• DOI: 10.14930/jsma1939.50.368

Evaluation of the severity of hone atrophy was attempted using the microdensitometry/multiple scanning (MD/MS) method to determine the morbidity of osteoporosis. To study quantitative and qualitative changes occurring in osteocalcin (OC), pure OC was refined by a new method with a good yield. The subjects were 75 cases of osteoporosis. Severity was determined by the MD/MS method, and the images obtained were evaluated according to Jikei's classfication by dividing them into 4 categories. Relations of blood Ca, P and AI-p with bone densities determined by the MD and MD/MS methods were investigated by measuring OC in blood. The method of Price et al. was modified by use of the femoral cortical bones in cattle and man, and OC was refined by high-pressure liquid chromatography (HPLC) . Result of studies suggest that the MD/MS method is useful as an index for evaluating osteoporosis, since classification by the MD/MS method correlates with Jikei's classification. Bone density tends to remain if OC is maintained, and the MD/MS method shows blood OC increasing in reaction to the decrease in bone density. This suggests that OC participates in bone formation. It has thus become possible to extract OC efficiently and simply, from which it is presumed that the new method will be useful in the future for determining the presence or absence of qualitative changes of OC.