脳腫瘍の細胞動態解析

• Published in: Neuro-Oncologyの進歩 Vol. 25; no. 2; pp. 1 - 7
• Main Authors: 黒瀬, 顕, 小川, 薫
• Format: Journal Article
• Language: English
• Published: 近畿脳腫瘍病理検討会 2018; Kinki Brain Tumor Pathology Conference
• Subjects: cell cycle; cell kinetics; cell proliferation; flow cytometry
• ISSN: 1880-0742; 2187-0551
• DOI: 10.11452/neurooncology.25.2_1

Brain tumors have been major and challenging subjects of cell kinetics study engaging many neurosurgeons and pathologists in the study of cytometry for its clinical significance. Counting mitotic figures has remained reliable to estimate malignant potential even in recent study. In cytometry, proliferative property was estimated first by a ratio of S phase cells which were detected using 3 H-thymidine or BrdU. Proliferative property estimated by S phase fraction was better concordant with prognosis compared to pathological diagnosis. Development of flow cytometry (FCM) facilitated automated calculation of proliferative indices and DNA ploidy patterns. Proliferative index correlated with histological malignancy, recurrence and invasion. DNA aneuploidy was proven to be malignant. Immunohistochemically, proliferative property had been estimated using anti-Ki-67 and anti-PCNA antibodies. Clone MIB1 has been used recently as the most reliable antibody to estimate proliferative property of tumors since its introduction. Multicolor FCM can provide useful data of protein expression corresponding to cell cycle phases. FISH and CGH have also aided the field of cytometry and are indispensable for genetic study. Such cytometric study of brain tumors provides dynamic and visualized cell kinetics.