Prolonged Recovery of Hematopoiesis after BMT in a Case of Severe Aplastic Anemia

• Published in: The Japanese Journal of Pediatric Hematology Vol. 10; no. 6; pp. 438 - 441
• Main Authors: NAKAYAMA, Masahiko, MATSUMOTO, Tadashi, KONDOH, Tatsuro, MATSUMOTO, Kazuhiro, TSUJI, Yoshiro, KAMITAMARI, Akira, MATSUYAMA, Takaharu, KUSUYAMA, Misako
• Format: Journal Article
• Language: Japanese
• Published: THE JAPANESE SOCIETY OF PEDIATRIC HEMATOLOGY/ONCOLOGY 1996; 特定非営利活動法人 日本小児血液・がん学会
• Subjects: bone marrow transplantation (BMT); polymorphic examination; prolongation of BM recovery; severe aplastic anemia (SAA)
• ISSN: 0913-8706; 1884-4723
• DOI: 10.11412/jjph1987.10.438

We performed bone marrow transplantation (BMT) on a male patient with severe aplastic anemia (SAA) who had received blood and/or platelet transfusions many times because of a resistance to all treatments. The total volume of transfusions was 34, 600ml. As the conditioning for BMT, CY, TLI and TBI were used. About 10 months was required for the reproliferation of bone marrow (BM) cells, including red cells, leukocytes and platelets. To confirm whether the condition of his BM cells was chimeric or had transferred to the donor type completely, we conducted a polymorphic examination using genomic DNA extracted from the peripheral leukocytes of his family and from his hair root cells. The results indicated that his BM cells had changed to donor-type cells completely. The possibilities of his prolonged hematopoietic recovery were as follows : (1) a long period of time is required to improve the microenvironment surrounding the BM; (2) the chimeric condition of donor-type cells and recipient cells continues after BMT, and requires a long period of time to change to donor-type cells; and (3) both (1) and (2).