Rapid Analysis of Stable-HbA1c by High-Performance Liquid Chromatography

• Published in: Japanese Journal of Clinical Chemistry Vol. 25; no. 3; pp. 177 - 184
• Main Authors: Kawasaki, Riichi, Kitamura, Takashi, Kato, Yoshio, Tukada, Toshihiko, Doi, Keiko, Nakayama, Toshimasa, Kawakami, Keiko
• Format: Journal Article
• Language: Japanese
• Published: Japan Society of Clinical Chemistry 1996; 一般社団法人 日本臨床化学会
• Subjects: HPLC; HPLC法; labile-HbA1c (L-A1c); stable-HbA1c (S-A1c); 不安定型HbA1c(L-A1c); 安定型HbA1c(S-A1c)
• ISSN: 0370-5633; 2187-4077
• DOI: 10.14921/jscc1971b.25.3_177

We studied rapid analysis of stable-HbA1c (SA1c) by high-performance liquid chromatography (HPLC) on a non-porous cation-exchanger measuring 2.5μm in diameter, TSKgel Glyco-HSi. The HbA1c fraction separated on a conventional cation-exchanger, TSKgel Glyco-HS, was resolved into more than four fractions on TSKgel Glyco-HSi, and the main component of them was S-A1c and minors were labile-HbA1c (L-A1c) and unknown components. The S-A1c concentrations in blood obtained from a patient undergoing 100g oral glucose tolerance test (OGTT) were not altered over the course of the test (0 to 180 min), whereas L-A1c levels markedly increased in blood with higher glucose concentrations. The normal reference values obtained for S-A1c and L-A1c, were 4.0-4.6% and 1.5-2.4%, respectively. S-A1c could be analyzed in about 2 min by the fullautomated glycated hemoglobin analyzer, HLC-723GHb III (TOSOH). Coefficient of variation (C. V.) for the S-A1c value was less than 1.2%.