A Simple Device for the Multi-Parallel Detection Method in the Liquid Chromatography and its Application for the Analysis of Catecholamines

• Published in: Proceedings of the Symposium on Chemical Physiology and Pathology Vol. 19; pp. 70 - 75
• Main Authors: AKIMOTO, Motohisa, YOSHIDA, Hisanobu, NAKAJIMA, Terumi, KITO, Shozo
• Format: Journal Article
• Language: Japanese
• Published: Japan Society of Clinical Chemistry 1980; 一般社団法人 日本臨床化学会
• ISSN: 0386-3417; 2187-4085
• DOI: 10.14921/jscc1971a.19.0_70

In order to identify the eluate, or to identify the series of metabolites of different chemical characteristics by a single liquid chromatography, it is awaited to establish the method to analyze the eluate by reasonable and different analytical methods at once. In this paper a simple device for the multi-parallel detection method in the liquid chromatography is presented. The device was made of multi-way joint (flow separator) and pressure coils. Pressure coils were contributed for the constant separation of the flow. Suppose that after the separation of the flow, one of the flow line was subjected to the analytical reaction using suction pump and another flow line was subjected analytical reaction under the back pressure. Then, the pressure coils were needed to minimize the change of flow rate in each line due to the different pressure in the different flow line. One of the best merit of this method was that each detection method worked at it's best sensitivity evenso the eluate was separated into several lines. Of cause, the flow separator separates the absolute amount of the eluate, but at before and after the separation, the concentration of the eluate should be the same. This is the reason that the detection sensitivity should be able to be the same with both the multi-parallel detection method and the single detection method. Another merit of this method is that wide range of analytical methods are able to be used with almost no restriction, for each flow line was devided independently by the pressure coil, in contrast to the multi-series detection method. Application of the multi-parallel detection method for the analysis of catecholamines, using detection methods of the trihydroxyindole (THI) method, o-phthalaldehyde (OPA) method and the electro-chemical method, is presented.