A Solid-Phase Enzymeimmunoassay for Human Albumin

• Published in: Japanese Journal of Clinical Chemistry Vol. 15; no. 2; pp. 80 - 84
• Main Authors: HIRATA, MEISEI, ISOBE, JUNICHI, MIZUNO, YOUKO, ENDOU, YUKIKO, SHOUNO, KAZUKO, SHIMA, KENJI, GOTOU, MASARU
• Format: Journal Article
• Language: Japanese
• Published: Japan Society of Clinical Chemistry 1986; 一般社団法人 日本臨床化学会
• ISSN: 0370-5633; 2187-4077
• DOI: 10.14921/jscc1971b.15.2_80

An enzymeimmunoassay (EIA) was established for measuring human urinary albumin. The assay was based on a two-step sandwitch method by using polystyrene beads as the solid-phase. Measurement covered from 5 to 1,000 ng/ml, and its coefficients of veriation of intraassay and interassay were 5.0-9.0% and 8.0-12.4% respectively. Dilution test showed a good linearity in the range of 0.1-1.0 O.D., and the recovery ratio was between 96-124%, This EIA showed a good correlation with an commercially available RIA system (r=0.974, n=78). The urinary albumin of normal and diabetic subjects, free of proteinuria by testtape method, was 7.12±3.96 and 31.87±52.72 mg/g·cr respectively, and the latter group showed a significantly (p<0.025) higher urinary albumin. This EIA could be a useful system for detecting trace amount of urinary albumin and be applied to the diagnosis of early renal dysfunction.