Usefulness of RT-PCR in Follow Up of a Case with Acute Lymphoblastic Leukemia with 1;19 Translocation
A 4-year-old girl was diagnosed in June 1990 as having acute lymphoblastic leukemia (ALL) with (1;19) translocation. Complete remission was obtained with TCCSG L84-12 intermediate risk protocol. Seven months after reinduction therapy, 50% of bone marrow cells showed blast-like morphology, with CD 10...
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| Published in | The Japanese Journal of Pediatric Hematology Vol. 7; no. 1; pp. 67 - 70 |
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| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | Japanese |
| Published |
THE JAPANESE SOCIETY OF PEDIATRIC HEMATOLOGY/ONCOLOGY
1993
特定非営利活動法人 日本小児血液・がん学会 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0913-8706 1884-4723 |
| DOI | 10.11412/jjph1987.7.67 |
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| Abstract | A 4-year-old girl was diagnosed in June 1990 as having acute lymphoblastic leukemia (ALL) with (1;19) translocation. Complete remission was obtained with TCCSG L84-12 intermediate risk protocol. Seven months after reinduction therapy, 50% of bone marrow cells showed blast-like morphology, with CD 10, 19, 20, HLA-DR antigen expression. In order to clarify whether the blast-like cells represent leukemic relapse or not, E2A-PBX1 chimeric mRNA was analyzed with RT-PCR. It showed only leukemic cell with (1;19) translocation. E2A-PBX1 transcript, which was expressed in the initial presentation sample, was not detected in those blast-like cells. Subsequently the patient was diagnosed as being in complete remisson. In this case, the continuous complete remission has been confirmed by the negativity of a serial E2A-PBX1 expression during treatment period. |
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| AbstractList | A 4-year-old girl was diagnosed in June 1990 as having acute lymphoblastic leukemia (ALL) with (1;19) translocation. Complete remission was obtained with TCCSG L84-12 intermediate risk protocol. Seven months after reinduction therapy, 50% of bone marrow cells showed blast-like morphology, with CD 10, 19, 20, HLA-DR antigen expression. In order to clarify whether the blast-like cells represent leukemic relapse or not, E2A-PBX1 chimeric mRNA was analyzed with RT-PCR. It showed only leukemic cell with (1;19) translocation. E2A-PBX1 transcript, which was expressed in the initial presentation sample, was not detected in those blast-like cells. Subsequently the patient was diagnosed as being in complete remisson. In this case, the continuous complete remission has been confirmed by the negativity of a serial E2A-PBX1 expression during treatment period. A 4-year-old girl was diagnosed in June 1990 as having acute lymphoblastic leukemia (ALL) with (1;19) translocation. Complete remission was obtained with TCCSG L84-12 intermediate risk protocol. Seven months after reinduction therapy, 50% of bone marrow cells showed blast-like morphology, with CD 10, 19, 20, HLA-DR antigen expression. In order to clarify whether the blast-like cells represent leukemic relapse or not, E2A-PBX1 chimeric mRNA was analyzed with RT-PCR. It showed only leukemic cell with (1;19) translocation. E2A-PBX1 transcript, which was expressed in the initial presentation sample, was not detected in those blast-like cells. Subsequently the patient was diagnosed as being in complete remisson. In this case, the continuous complete remission has been confirmed by the negativity of a serial E2A-PBX1 expression during treatment period. 4歳, 女児.1990年6月発症の (1;19) 転座を有するpre-BALL. TCCSGのALLL84-12 intermediatedriskに対するプロトコールにて完全寛解がえられた.7カ月後の再寛解導入療法直後の骨髄穿刺標本にて, 芽球様細胞が50%をしめた.表面マーカーはCD10, 19, 20, HLA-DR陽性であったため, 再発が疑われた.そこで, RT-PCR法を用いたE2A-PBX1遺伝子の解析を行い, 再発でないことを証明した.その後も継時的にPCR法にて観察して, 寛解を維持していることを確認している. |
| Author | HAYASHI, Yasuhide OHARA, Akira KOJIMA, Kyouko NAKAZAWA, Sinnpei SAWA, Fumihiro TUUKIMOTO, Itirou MIZUTANI, Syuki SUZUKI, Toshio OKAMOTO, Norihiko SAWAI, Kiyoshi |
| Author_FL | 沢井 清 水谷 修紀 KOJIMA Kyouko 岡本 則彦 SAWA Fumihiro 鈴木 敏雄 中沢 真平 月本 一郎 小原 明 林 泰秀 |
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| References | 7) Nourse J, Mellentin JD, Galili N, et al : Chromosomal translocation t (1; 19) results in synthesis of a homeobox fusion mRNA that codes for a potential chimeric transcription factor. Cell 60 : 535-545, 1990 3) Carroll AJ, Crist WM, Parmley KT, et al : Pre-B cell leukemia associated with chromosome translocation 1; 19. Blood 63 : 721-724, 1984 4) Crist WM, Carroll AJ, Shuster FG, et al : Poor prognosis of children with pre-B acute lymphoblastic leukemia is associated with the t (1; 19) (q23; p13) : A Pediatric Oncology Group Study. Blood 76 : 117-122, 1990 2) Hunger SP, Galili N, Carroll AJ, et al : The t (1; 19) (q23; p13) results in consistent fusion of E2A and PBX1 coding sequences in acute lymphoblastic leukemias. Blood 77 : 687-693, 1991 6) Kamps MP, Murre C, Sun X, et al : A new homeobox gene contributes the DNA binding domain of the t (1; 19) translocation protein in pre-B ALL. Cell 60 : 547-555, 1990 5) 清井仁, 市橋卓司, 福谷久, 他 : B細胞系腫瘍における免疫グロブリンH鎖遺伝子超可変部領域の構造解析と微少残存細胞検出への応用.日血会誌54 (補冊1) : 141, 1991 1) Tokyo Children's Cancer Study Group : TCCSG L89-12 Protcol for ALL, 2nd Ed, Oct. 1990 |
| References_xml | – reference: 5) 清井仁, 市橋卓司, 福谷久, 他 : B細胞系腫瘍における免疫グロブリンH鎖遺伝子超可変部領域の構造解析と微少残存細胞検出への応用.日血会誌54 (補冊1) : 141, 1991 – reference: 1) Tokyo Children's Cancer Study Group : TCCSG L89-12 Protcol for ALL, 2nd Ed, Oct. 1990 – reference: 3) Carroll AJ, Crist WM, Parmley KT, et al : Pre-B cell leukemia associated with chromosome translocation 1; 19. Blood 63 : 721-724, 1984 – reference: 4) Crist WM, Carroll AJ, Shuster FG, et al : Poor prognosis of children with pre-B acute lymphoblastic leukemia is associated with the t (1; 19) (q23; p13) : A Pediatric Oncology Group Study. Blood 76 : 117-122, 1990 – reference: 7) Nourse J, Mellentin JD, Galili N, et al : Chromosomal translocation t (1; 19) results in synthesis of a homeobox fusion mRNA that codes for a potential chimeric transcription factor. Cell 60 : 535-545, 1990 – reference: 6) Kamps MP, Murre C, Sun X, et al : A new homeobox gene contributes the DNA binding domain of the t (1; 19) translocation protein in pre-B ALL. Cell 60 : 547-555, 1990 – reference: 2) Hunger SP, Galili N, Carroll AJ, et al : The t (1; 19) (q23; p13) results in consistent fusion of E2A and PBX1 coding sequences in acute lymphoblastic leukemias. Blood 77 : 687-693, 1991 |
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| SubjectTerms | ALL with 1, 19 translocation E2A-PBX1 oncogene RT-TCR |
| Title | Usefulness of RT-PCR in Follow Up of a Case with Acute Lymphoblastic Leukemia with 1;19 Translocation |
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