Usefulness of RT-PCR in Follow Up of a Case with Acute Lymphoblastic Leukemia with 1;19 Translocation

• Published in: The Japanese Journal of Pediatric Hematology Vol. 7; no. 1; pp. 67 - 70
• Main Authors: OKAMOTO, Norihiko, KOJIMA, Kyouko, OHARA, Akira, NAKAZAWA, Sinnpei, SAWAI, Kiyoshi, HAYASHI, Yasuhide, MIZUTANI, Syuki, TUUKIMOTO, Itirou, SUZUKI, Toshio, SAWA, Fumihiro
• Format: Journal Article
• Language: Japanese
• Published: THE JAPANESE SOCIETY OF PEDIATRIC HEMATOLOGY/ONCOLOGY 1993; 特定非営利活動法人 日本小児血液・がん学会
• Subjects: ALL with 1, 19 translocation; E2A-PBX1 oncogene; RT-TCR
• ISSN: 0913-8706; 1884-4723
• DOI: 10.11412/jjph1987.7.67

A 4-year-old girl was diagnosed in June 1990 as having acute lymphoblastic leukemia (ALL) with (1;19) translocation. Complete remission was obtained with TCCSG L84-12 intermediate risk protocol. Seven months after reinduction therapy, 50% of bone marrow cells showed blast-like morphology, with CD 10, 19, 20, HLA-DR antigen expression. In order to clarify whether the blast-like cells represent leukemic relapse or not, E2A-PBX1 chimeric mRNA was analyzed with RT-PCR. It showed only leukemic cell with (1;19) translocation. E2A-PBX1 transcript, which was expressed in the initial presentation sample, was not detected in those blast-like cells. Subsequently the patient was diagnosed as being in complete remisson. In this case, the continuous complete remission has been confirmed by the negativity of a serial E2A-PBX1 expression during treatment period.