A Comparative Study of Recent Assays for the Determin8tion of Serum L-Cystine-aminopeptidase(CAP)
The authors describe a simplified and rapid method for determining L-cystineaminopeptidase ( CAP) in serum with the aid of S-benzyl-L-cysteine-p-nitroanilide (BCN). The liberated p-nitroaniline reacts with p-dimethylaminocinnamaldehyde to give ared dye. The method requires 15min incubation at 37°, f...
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| Published in | Japanese Journal of Clinical Chemistry Vol. 5; no. 1; pp. 83 - 90 |
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| Main Authors | , |
| Format | Journal Article |
| Language | Japanese |
| Published |
Japan Society of Clinical Chemistry
1976
一般社団法人 日本臨床化学会 |
| Online Access | Get full text |
| ISSN | 0370-5633 2187-4077 |
| DOI | 10.14921/jscc1971b.5.1_83 |
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| Summary: | The authors describe a simplified and rapid method for determining L-cystineaminopeptidase ( CAP) in serum with the aid of S-benzyl-L-cysteine-p-nitroanilide (BCN). The liberated p-nitroaniline reacts with p-dimethylaminocinnamaldehyde to give ared dye. The method requires 15min incubation at 37°, followed by 10min colour development. The proposed method is more sensitive than that with using L-cystine-di-P-nitroaniiinde (CDNl) and using L-cystine-di-naphthylamide (Wada's method). The correlation coefficient between results of this method and those obtained with L-cystine-di-naphthylamide (CDNA) as substrate was 0.97. |
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| ISSN: | 0370-5633 2187-4077 |
| DOI: | 10.14921/jscc1971b.5.1_83 |