Safety Handling of 125I in Animal Experiments

Isotope 125I in animal experiments is used for several purposes. One major problem with using 125I is to handle it extremely carefully. The careful handling is required when using 125I in animal experiments because of its higher evaporation and difficulty for sealing. When conducting animal experime...

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Bibliographic Details
Published inRADIOISOTOPES Vol. 45; no. 10; pp. 626 - 630
Main Authors NISHIMAKI, Toshiyuki, FURUDATE, Senichi, SAKAI, Yasuhiro
Format Journal Article
LanguageJapanese
Published Japan Radioisotope Association 15.10.1996
Subjects
animal experiment
autoradiography
iodine-125
safety handling
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ISSN0033-8303
1884-4111
DOI10.3769/radioisotopes.45.626

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Summary:Isotope 125I in animal experiments is used for several purposes. One major problem with using 125I is to handle it extremely carefully. The careful handling is required when using 125I in animal experiments because of its higher evaporation and difficulty for sealing. When conducting animal experiments while using 125I, we have encountered several serious problems but have devised new techniques and methods for a long time. In this paper, we will describe the safety handling for requirements based on our experiments. The newly devised safety handling procedures are the following : (1) a device for protecting isotope contamination during the preparation of the injectable solution, (2) collection method of airborne radioactivity from the animals that were injected, (3) enhancing the method for autoradiography of the whole body, (4) finding of elusion of 125I from the tissues during fixative process, (5) estimation of the exposed time for light microscopic autoradiography.
ISSN:0033-8303
1884-4111
DOI:10.3769/radioisotopes.45.626