ダイオードアレイ検出器と荷電化粒子検出器を用いたLC-NMRシステムの構築

Nuclear magnetic resonance (NMR) spectroscopy is a powerful tool for structure determination. However, NMR spectra sometimes suffer from severe signal overlaps. To avoid such signal overlaps, LC-DAD-NMR systems have been used. These systems have enabled the separation of components on a column befor...

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Bibliographic Details
Published inBunseki kagaku Vol. 69; no. 3; p. 151
Main Authors KANBAYASHI, Yayoi, TSUTSUMI, Yu, MIZUKOSHI, Toshimi, YAMAGUCHI, Hideyuki
Format Journal Article
LanguageJapanese
Published Tokyo Japan Science and Technology Agency 05.03.2020
Subjects
Aerosols
Chromophores
Fructose
Glucose
Mathematical analysis
NMR
Nuclear magnetic resonance
Sensors
Separation
Spectroscopy
Spectrum analysis
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ISSN0525-1931
DOI10.2116/bunsekikagaku.69.151

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Summary:Nuclear magnetic resonance (NMR) spectroscopy is a powerful tool for structure determination. However, NMR spectra sometimes suffer from severe signal overlaps. To avoid such signal overlaps, LC-DAD-NMR systems have been used. These systems have enabled the separation of components on a column before NMR detection. However, DAD detection alone is insufficient when the sample does not contain chromophores. To increase the detection coverage, we used a corona charged aerosol detector (CAD) that responded to analytes independent of their physical/chemical characteristics. The established LC-DAD/CAD NMR system was evaluated. Its calculated detection and quantitation limits were 0.25 and 1.0 μg/peak, respectively. This system was used to analyze rubus extract. Along with glucose and fructose, a small amount of rubusoside was detected by corona CAD and identified by NMR without needing a chromophore.
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ISSN:0525-1931
DOI:10.2116/bunsekikagaku.69.151