Effect of Short Storage Time on Concentration of Interleukin-6 in Plasma Samples

• Published in: Transactions of Japanese Society for Medical and Biological Engineering Vol. Annual59; no. Abstract; p. 401
• Main Authors: Sugano, Mitsutoshi, Koizumi, Tomonobu, Yamaguchi, Masaki
• Format: Journal Article
• Language: Japanese
• Published: Japanese Society for Medical and Biological Engineering 2021; 公益社団法人 日本生体医工学会
• ISSN: 1347-443X; 1881-4379
• DOI: 10.11239/jsmbe.Annual59.401

Interleukin-6 (IL-6) is potentially useful biomarker of conditions requiring emergency transport or intensive care. However, IL-6 can be released by leukocytes and platelets after sampling. We examined the effects of short storage time on concentration of IL-6 in plasma samples. The study protocol was approved by the ethical committee. Venous bloods were collected from twelve healthy male subjects (34.8 yr) by a vacuum sample EDTA tube. Immediately after, the blood samples were centrifuged at 2,000 G. The IL-6 was analyzed using an IL-6 kit (Elecsys, Roche Diagnostics K.K.) and an analyzer (cobas8000). The IL-6 levels showed lower values than limit of detection of 1.5 pg/mL in 11 subjects. These values were low levels compared to the previously reported 4.98 pg/mL (66 normal subjects). It was concluded that the lowest values for IL-6 was found in EDTA samples, centrifuged immediately after blood collection and thereafter stored on crushed ice.