Mitochondrial bioenergetics, mass, and morphology are altered in cells of the degenerating human annulus

• Published in: Journal of orthopaedic research Vol. 31; no. 8; pp. 1270 - 1275
• Main Authors: Gruber, Helen E., Watts, John A., Riley, Frank E., Fulkerson, Mary-Beth, Norton, H. James, Hanley Jr, Edward N.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.08.2013
• Subjects: Adult; Aged; Aged, 80 and over; annulus; bioenergetics; Cells, Cultured; Citrate (si)-Synthase - metabolism; disc degeneration; Energy Metabolism - physiology; Female; Humans; Intervertebral Disc - metabolism; Intervertebral Disc - pathology; Intervertebral Disc - ultrastructure; Intervertebral Disc Degeneration - complications; Intervertebral Disc Degeneration - metabolism; Intervertebral Disc Degeneration - pathology; Intervertebral Disc Displacement - complications; Intervertebral Disc Displacement - metabolism; Intervertebral Disc Displacement - pathology; Male; Microscopy, Electron, Transmission; Middle Aged; Mitochondria - enzymology; Mitochondria - pathology; Mitochondria - ultrastructure; mitochondrial respiration; Mitochondrial Size; Oxidative Stress; Oxygen Consumption; Prospective Studies; Young Adult
• ISSN: 0736-0266; 1554-527X; 1554-527X
• DOI: 10.1002/jor.22361

Back pain and intervertebral disc degeneration have a growing socioeconomic healthcare impact. Information on mitochondrial function in human intervertebral disc cells, however, is surprisingly sparse. We assessed mitochondrial bioenergetics, mass, and ultrastructure in annulus cells cultured from human discs of varying degenerative stages. Citrate synthase activity (reflecting mitochondrial mass) declined significantly with increasing Thompson grade (p < 0.0001). Both mitochondrial (p = 0.009) and non‐mitochondrial (p = 0.0029) respiration showed significant changes with increasing stages of disc degeneration. No significant relationships were found for the association of respiration data with herniated or non‐herniated status, or with subject age. Examination of mitochondrial ultrastructure in cultured annulus cells revealed unusual features which included mitochondrial inclusion bodies, poorly defined cristae and dark staining. Findings reported here are novel and document biochemical, metabolic, and morphologic abnormalities in mitochondria in cells from more degenerated annulus cells. Data suggest that the disc degenerative, not age, is a major factor associated with mitochondrial impairment, and also implicate oxidative stress, driven by mitochondrial dysfunction, as a major component within the degenerating disc. Findings have relevance to advancements in cell‐based therapies to treat disc degeneration. © 2013 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 31:1270–1275, 2013