Comparison of a gel column blood typing method and a point-of-care cartridge for Dog Erythrocyte Antigen 1.1
Background Blood typing for the presence of Dog Erythrocyte Antigen (DEA) 1.1 is recommended in all donor and recipient dogs prior to transfusion of blood products. The objective of this study was to determine if a point‐of‐care DEA 1.1 blood typing cartridge could be used in place of the gel column...
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Published in | Journal of veterinary emergency and critical care (San Antonio, Tex. : 2000) Vol. 23; no. 3; pp. 340 - 343 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
Blackwell Publishing Ltd
01.05.2013
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Subjects | |
Online Access | Get full text |
ISSN | 1479-3261 1476-4431 1476-4431 |
DOI | 10.1111/vec.12052 |
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Summary: | Background
Blood typing for the presence of Dog Erythrocyte Antigen (DEA) 1.1 is recommended in all donor and recipient dogs prior to transfusion of blood products. The objective of this study was to determine if a point‐of‐care DEA 1.1 blood typing cartridge could be used in place of the gel column typing method.
Study Design
Detection of DEA 1.1 was performed using a laboratory‐based gel column method and a point‐of‐care cartridge. A convenience sample of 30 healthy blood donors, 13 dogs with immune‐mediated hemolytic anemia (IMHA) (3 of which had concurrent immune‐mediated thrombocytopenia [IMT]), and 44 dogs with other diseases was included in the study.
Key Findings
Agreement was observed between the tests for normal dogs and dogs with nonimmune‐mediated disease in 74/74 cases. Two dogs in the IMHA group had indeterminate gel column blood typing results; 1 dog in this group had a negative gel column test result but a positive cartridge test result.
Significance
There was good agreement between the 2 methods for normal dogs and dogs with nonimmune‐mediated disease. Blood typing methods in dogs with IMHA should be further investigated. |
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Bibliography: | istex:519AB1064062D6FDEF77A23E82A9D44E0DD32723 Ontario Veterinary College Pet Trust Fund ArticleID:VEC12052 ark:/67375/WNG-LMBMQ2KM-M The authors declare no conflict of interests. Financial support for this study provided by the Ontario Veterinary College Pet Trust Fund. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1479-3261 1476-4431 1476-4431 |
DOI: | 10.1111/vec.12052 |