Comparison of a gel column blood typing method and a point-of-care cartridge for Dog Erythrocyte Antigen 1.1

• Published in: Journal of veterinary emergency and critical care (San Antonio, Tex. : 2000) Vol. 23; no. 3; pp. 340 - 343
• Main Authors: Blois, Shauna L., Richardson, Danielle M., Abrams-Ogg, Anthony C. G.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.05.2013
• Subjects: Anemia, Hemolytic, Autoimmune - blood; Anemia, Hemolytic, Autoimmune - veterinary; Animals; Antigens - blood; Blood Donors; Blood Grouping and Crossmatching - methods; Blood Grouping and Crossmatching - veterinary; canine; Dog Diseases - blood; Dogs - blood; Point-of-Care Systems; Purpura, Thrombocytopenic, Idiopathic - blood; Purpura, Thrombocytopenic, Idiopathic - veterinary; transfusion medicine; transfusion reactions
• ISSN: 1479-3261; 1476-4431; 1476-4431
• DOI: 10.1111/vec.12052

Background Blood typing for the presence of Dog Erythrocyte Antigen (DEA) 1.1 is recommended in all donor and recipient dogs prior to transfusion of blood products. The objective of this study was to determine if a point‐of‐care DEA 1.1 blood typing cartridge could be used in place of the gel column typing method. Study Design Detection of DEA 1.1 was performed using a laboratory‐based gel column method and a point‐of‐care cartridge. A convenience sample of 30 healthy blood donors, 13 dogs with immune‐mediated hemolytic anemia (IMHA) (3 of which had concurrent immune‐mediated thrombocytopenia [IMT]), and 44 dogs with other diseases was included in the study. Key Findings Agreement was observed between the tests for normal dogs and dogs with nonimmune‐mediated disease in 74/74 cases. Two dogs in the IMHA group had indeterminate gel column blood typing results; 1 dog in this group had a negative gel column test result but a positive cartridge test result. Significance There was good agreement between the 2 methods for normal dogs and dogs with nonimmune‐mediated disease. Blood typing methods in dogs with IMHA should be further investigated.