α-Tocopherol Dietary Supplement Decreases Titers of Antibody against 5-Hydroxymethyl-2′-Deoxyuridine (HMdU)
This study evaluated the effects of vitamin E (α-tocopherol) on oxidative DNA damage in a randomized double-blind Phase II chemoprevention trial. Oxidative DNA damage was measured by the level of auto-antibody (Ab) against 5-hydroxymethyl-2′-deoxyuridine (HMdU) in plasma. After the baseline screenin...
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Published in | Cancer epidemiology, biomarkers & prevention Vol. 8; no. 8; pp. 693 - 698 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Philadelphia, PA
American Association for Cancer Research
01.08.1999
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Subjects | |
Online Access | Get full text |
ISSN | 1055-9965 1538-7755 |
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Summary: | This study evaluated the effects of vitamin E (α-tocopherol) on oxidative DNA damage in a randomized double-blind Phase II
chemoprevention trial. Oxidative DNA damage was measured by the level of auto-antibody (Ab) against 5-hydroxymethyl-2′-deoxyuridine
(HMdU) in plasma. After the baseline screening, eligible subjects ( n = 31; plasma samples from 28 subjects were available for this study) were randomized to receive 15, 60, or 200 mg of α-tocopherol
per day for 28 days. Biomarkers were measured twice at baseline—on day 1 (visit 1) and day 3 (visit 2)—and twice after intervention—on
day 17 (visit 3) and day 31 (visit 4). At baseline, there was a highly significant inverse correlation between anti-HMdU Ab
titer and plasma vitamin E level ( r = −0.53; P = 0.004; n = 28). Smoking did not affect baseline anti-HMdU Ab titer; however, anti-HMdU Ab titer levels at baseline were significantly
lower in subjects with above-median (0.75 ounce/day) alcohol consumption ( P = 0.008). No significant change in anti-HMdU Ab level occurred at either visit 3 or visit 4 for subjects on the lowest dose,
15 mg α-tocopherol per day. Subjects receiving 60 mg of α-tocopherol per day had a significant decrease in anti-HMdU Ab level
at visits 3 and 4 compared with baseline ( P = 0.049 and P = 0.02, respectively). However, subjects receiving the highest dose, 200 mg/day, had less consistent results: a significant
decrease in anti-HMdU Ab level was seen at visit 4 ( P = 0.04) but not at visit 3. Our results demonstrate an inverse relationship between α-tocopherol and anti-HMdU Abs in plasma;
oxidative DNA damage can be modulated by short-term dietary supplementation of α-tocopherol in some subjects. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Undefined-3 |
ISSN: | 1055-9965 1538-7755 |