Expression of Gene rrg is Associated with Reversion of NIH 3T3 Transformed by LTR-c-H-ras

• Published in: Science (American Association for the Advancement of Science) Vol. 249; no. 4970; pp. 796 - 798
• Main Authors: Contente, Sara, Kenyon, Kaylene, Rimoldi, Donata, Friedman, Robert M.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 17.08.1990; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: 3T3 cells; Animals; BALB 3T3 cells; Biological and medical sciences; Cell Line, Transformed; Cell lines; Cell physiology; Cell transformation; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes; Cloning, Molecular; Complementary DNA; Deoxyribonucleic acid; DNA; DNA - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression; Genes, ras; Genetics; Humans; Interferon Type I - pharmacology; Mice; Mice, Nude; Molecular and cellular biology; Neoplasms, Experimental - etiology; NIH 3T3 cells; Nucleic Acid Hybridization; Oncogenes; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins p21(ras); Rats; RNA; RNA - analysis; RNA - genetics; RNA, Antisense; RNA, Messenger - genetics; Rodents; Transfection; Transformed cell line; Tumor cell line; Tumors; Molecular hybridization; 3T3»-Cell line; Rodentia; Gene expression; Cell transformation; Vertebrata; Reversion; Mammalia; Cell line; Transfection; Complementary DNA; Mouse; C-Onc gene; Inhibition
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1697103

A partial complementary DNA was isolated for a gene (rrg) that is normally expressed in mouse NIH 3T3 cells, but is down-regulated after cellular transformation by long terminal repeat (LTR)-activated c-H-ras (LTR-c-H-ras). This gene was reexpressed in a nontumorigenic persistent revertant cell line created by prolonged treatment of the transformed cells with mouse interferon α/β. Persistent revertants stably transfected with rrg complementary DNA antisense expression vectors appeared transformed, had decreased amounts of rrg messenger RNA, and were tumorigenic in nude mice. Stable transfection with sense constructs did not alter the normal morphology, message level, or nontumorigenicity of the persistent revertant cell line.