Antibacterial, antioxidant, anti-cholinesterase potential and flavonol glycosides of Biscutella raphanifolia (Brassicaceae)

• Published in: Pakistan journal of pharmaceutical sciences Vol. 28; no. 1; p. 153
• Main Authors: Boudouda, Houria Berhail, Zeghib, Assia, Karioti, Anastazia, Bilia, Anna Rita, Öztürk, Mehmet, Aouni, Mahjoub, Kabouche, Ahmed, Kabouche, Zahia
• Format: Journal Article
• Language: English
• Published: Pakistan Pakistan Journal of Pharmaceutical Sciences 01.01.2015
• Subjects: 1-Butanol - chemistry; Acetates - chemistry; Acetylcholinesterase - metabolism; Alkanes - chemistry; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Bacteria - drug effects; Bacteria - growth & development; Botanical research; Brassicaceae; Brassicaceae - chemistry; Butyrylcholinesterase - metabolism; Cholinesterase Inhibitors - chemistry; Cholinesterase Inhibitors - isolation & purification; Cholinesterase Inhibitors - pharmacology; Flavonols - chemistry; Flavonols - isolation & purification; Flavonols - pharmacology; Glycosides; Glycosides - chemistry; Glycosides - isolation & purification; Glycosides - pharmacology; Health aspects; Methylene Chloride - chemistry; Phytotherapy; Plant biochemistry; Plant Components, Aerial; Plant extracts; Plants, Medicinal; Properties; Solvents - chemistry
• ISSN: 1011-601X

Different extracts of the aerial parts of Biscutella raphanifolia (Brassicaceae), which has not been the subject of any study, were screened for the phytochemical content, anti-microbial, antioxidant and anti-cholinesterase activities. We used four methods to identify the antioxidant activity namely, ABTS(•+), DPPH• scavenging, CUPRAC and ferrous-ions chelating methods. Since there is a relationship between antioxidants and cholinesterase enzyme inhibitors, we used two methods to determine the in vitro anti-cholinesterase activity by the use of the basic enzymes that occur in causing Alzheimer's disease: acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). The extracts were also tested in vitro antimicrobial activity against various bacteria. The phytochemical study of B. raphanifolia afforded four flavonol glycosides; namely, quercetin-3-O-β-D-g1ucoside, quercetin-3-O-[β-D-glucosyl(1→2)-O-β-D-glucoside], quercetin-3-O-[β-D-glucosyl(1→3)-O-β-D-glucoside] and kaempferol-3-O-[β-D-glucosyl(1→2)-[(6'''p-coumaroyl)- β-D-glucoside], being isolated here for the first time from Biscutella raphanifolia and the genus. The ethyl acetate extract showed the highest activity in ABTS(•+), DPPH• and CUPRAC assays, while the petroleum ether extract demonstrated optimum efficiency metal chelating activity. The dicloromethane and petroleum ether extracts showed a mild inhibition against AChE and BChE. However, the petroleum ether extract showed a good antibacterial activity against the pathovars Enteropathogenic E. coli (EPEC), Enterotoxigenic E. coli (ETEC) and Enterococcus feacalis, whereas the Enterohemorrhagic E. coli (EHEC) strain was more sensitive to dichloromethane and n-butanol extracts.