Predominant genera of fecal microbiota in children with atopic dermatitis are not altered by intake of probiotic bacteria Lactobacillus acidophilus NCFM and Bifidobacterium animalis subsp. lactis Bi-07

• Published in: FEMS microbiology ecology Vol. 75; no. 3; pp. 482 - 496
• Main Authors: Larsen, Nadja, Vogensen, Finn K, Gøbel, Rikke, Michaelsen, Kim F, Al-Soud, Waleed Abu, Sørensen, Søren J, Hansen, Lars H, Jakobsen, Mogens
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.03.2011; Oxford University Press
• Subjects: atopic dermatitis; Bacteria; Bifidobacterium; Bifidobacterium - classification; Bifidobacterium - genetics; Bifidobacterium - physiology; Bifidobacterium adolescentis; Bifidobacterium animalis; Bifidobacterium animalis subsp. lactis; Bifidobacterium bifidum; Bifidobacterium catenulatum; Bifidobacterium lactis; Bifidobacterium longum; Biodiversity; Child; Child, Preschool; children; Cluster Analysis; cohort studies; correlation; denaturing gradient gel electrophoresis; Dermatitis; Dermatitis, Atopic - microbiology; Ecology; Eczema; fecal microbiota; Feces; Feces - microbiology; genes; Humans; Infant; Lactobacillus; Lactobacillus - classification; Lactobacillus - genetics; Lactobacillus - physiology; Lactobacillus acidophilus; Lactobacillus acidophilus - genetics; Lactobacillus acidophilus - physiology; Lactobacillus fermentum; Lactobacillus gasseri; Lactobacillus oris; Leuconostoc mesenteroides; Microbiology; placebos; probiotic bacteria; Probiotics; quantitative polymerase chain reaction; ribosomal RNA; RNA, Ribosomal, 16S - genetics; sequence analysis; Skin diseases; Yogurt; fecal microbiota; probiotic bacteria
• ISSN: 0168-6496; 1574-6941; 1574-6941
• DOI: 10.1111/j.1574-6941.2010.01024.x

The effect of probiotic bacteria Lactobacillus acidophilus NCFM and Bifidobacterium lactis Bi-07 on the composition of the Lactobacillus group, Bifidobacterium and the total bacterial population in feces from young children with atopic dermatitis was investigated. The study included 50 children randomized to intake of one of the probiotic strain or placebo. Microbial composition was characterized by denaturing gradient gel electrophoresis, quantitative PCR and, in a subset of subjects, by pyrosequencing of the 16S rRNA gene. The core population of the Lactobacillus group was identified as Lactobacillus gasseri, Lactobacillus fermentum, Lactobacillus oris, Leuconostoc mesenteroides, while the bifidobacterial community included Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium longum and Bifidobacterium catenulatum. The fecal numbers of L. acidophilus and B. lactis increased significantly after intervention, indicating survival of the ingested bacteria. The levels of Bifidobacterium correlated positively (P=0.03), while the levels of the Lactobacillus group negatively (P=0.01) with improvement of atopic eczema evaluated by the Severity Scoring of Atopic Dermatitis index. This correlation was observed across the whole study cohort and not attributed to the probiotic intake. The main conclusion of the study is that administration of L. acidophilus NCFM and B. lactis Bi-07 does not affect the composition and diversity of the main bacterial populations in feces.