Optimization of Culture Conditions and Bench-Scale Production of L-Asparaginase by Submerged Fermentation of Aspergillus terreus MTCC 1782

• Published in: Journal of microbiology and biotechnology Vol. 22; no. 7; pp. 923 - 929
• Main Authors: Gurunathan, Baskar, St. Joseph's College of Engineering, Chennai, India, Sahadevan, Renganathan, Anna University, Chennai, India
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.07.2012
• Subjects: Algorithms; artificial neural network; asparaginase; Asparaginase - biosynthesis; Aspergillus - enzymology; Aspergillus - growth & development; ASPERGILLUS TERREUS; bench-scale bioreactor; Biological and medical sciences; Bioreactors; Bioreactors - microbiology; Biotechnology; Fermentation; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; Methods. Procedures. Technologies; Neural Networks (Computer); response surface methodology; Temperature; Time Factors; Various methods and equipments; artificial neural network; bench-scale bioreactor; Enzyme; response surface methodology; Neural network; Optimization; Fungi; Bioreactor; Submerged culture; Production; Hydrolases; Response surface; Fungi Imperfecti; Aspergillus terreus; Asparaginase
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1112.12002

Optimization of culture conditions for L-asparaginase production by submerged fermentation of Aspergillus terreus MTCC 1782 was studied using a 3-level central composite design of response surface methodology and artificial neural network linked genetic algorithm. The artificial neural network linked genetic algorithm was found to be more efficient than response surface methodology. The experimental L-asparaginase activity of 43.29 IU/ml was obtained at the optimum culture conditions of temperature 35℃, initial pH 6.3, inoculum size 1% (v/v), agitation rate 140 rpm, and incubation time 58.5 h of the artificial neural network linked genetic algorithm, which was close to the predicted activity of 44.38 IU/ml. Characteristics of L-asparaginase production by A. terreus MTCC 1782 were studied in a 3 L bench-scale bioreactor.