An Antifungal Antibiotic Purified from Bacillus megaterium KL39, a Biocontrol Agent of Red-Pepper Phytophthora-Blight Disease

Bacillus megaterium KL39, an antibiotic-producing plant growth promoting rhizobacterium (PGPR), was selected from soil. The antifungal antibiotic, denoted KL39, was purified from culture filtrate by column chromatography using Dion HP-20, Silica gel, Sephadex LH-20, and prep-HPLC. Thin layer chromat...

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Bibliographic Details
Published inJournal of microbiology and biotechnology Vol. 15; no. 5; pp. 1001 - 1010
Main Authors Jung, H.K. (Yeungnam University, Gyeongsan, Republic of Korea), Kim, S.D. (Yeungnam University, Gyeongsan, Republic of Korea), E-mail: sdkim@yumail.ac.kr
Format Journal Article
LanguageEnglish
Published Seoul Korean Society for Applied Microbiology 01.10.2005
한국미생물·생명공학회
Subjects
Antifungal antibiotic
Biological and medical sciences
BIOLOGICAL CONTROL
Biotechnology
CHILI DULCE
CONTROL BIOLOGICO
Fundamental and applied biological sciences. Psychology
iturin E
LUTTE BIOLOGIQUE
phytophthora-blight
PIMENT DOUX
SWEET PEPPERS
생물학
Disease
Biological control
Phycomycetes
Bacillaceae
Antifungal antibiotic
Fungi
Antibiotic
Antifungal agent
phytophthora-blight
Bacillales
Bacteria
red-pepper
Phytophthora
biocontrol
Bacillus megaterium
Thallophyta
iturin E
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ISSN1017-7825

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Summary:Bacillus megaterium KL39, an antibiotic-producing plant growth promoting rhizobacterium (PGPR), was selected from soil. The antifungal antibiotic, denoted KL39, was purified from culture filtrate by column chromatography using Dion HP-20, Silica gel, Sephadex LH-20, and prep-HPLC. Thin layer chromatography, employing the solvent system of ethanol:ammonia:water=8:1:1, showed the R∧f value of 0.32. The antibiotic KL39 showed a negative reaction with ninhydrin solution, positive with iodine vapor, and also positive with Ehrlich reagent.
Bibliography:2006015294
F62
G704-000169.2005.15.5.005
ISSN:1017-7825