Selection and Physiological Characterization of Glyphosate-tolerant Zoysiagrass Mutants Derived from a Gamma Ray Irradiation

• Published in: Weon'ye gwahag gi'sulji Vol. 26; no. 4; pp. 454 - 463
• Main Authors: Lee, H.J. (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea), Lee, G.J. (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea), Kim, D.S. (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea), Kim, J.B. (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea), Ku, J.H. (Chungnam National University, Daejeon, Republic of Korea), Kang, S.Y. (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea), E-mail: sykang@kaeri.re.kr
• Format: Journal Article
• Language: English
• Published: 한국원예학회HST 01.12.2008; 한국원예학회
• Subjects: 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS); electrolyte leakage; glyphosate herbicide; malondealdehyde; MUTACION; MUTATION; pigment content; PROLINA; PROLINE; ZOYSIA JAPONICA; 농학; 돌연변이; 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS); electrolyte leakage; mutagenesis; 프로린; proline; glyphosate 제초제; Zoysia japonica; glyphosate herbicide; 전해질 유출; 색소함량; malondealdehyde; pigment content
• ISSN: 1226-8763; 2465-8588

This study was conducted to determine the successful in vitro and in vivo conditions for a mutant induction of zoysiagrass (Zoysia japonica), and to observe the physiological responses of the selected mutants resistant to a glyphosate herbicide. A mutation induction under in vitro conditions was found to be achievable when combined with the gamma ray in zoysiagrass (2 mgㆍL-¹ 2,4-D and 0.1 to 0.5 mgㆍL-¹ BA with 50-70 Gy of a gamma ray). The electrolyte leakage (EL), malondealdehyde (MDA) and proline contents increased, while phyto pigments decreased with increasing glyphosate concentration, especially with 0.5-1.0% glyphosate level from 7 days after treatment. After the leaf blade of 100 M2 plants derived from a gamma ray treatment (300 Gy) was sprayed, three resistant and susceptible plants were selected and used to compare the physiological responses to a 0.5% glyphosate treatment. The EL was increased more in the susceptible plants than in the resistant mutants, while the MDA content was not evident statistically. The chlorophyll and carotenoid contents decreased in all the treated materials, with less reduction in the susceptible lines. The activity of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was about 1.5-fold higher in the resistant mutants, allowing them resistance to glyphosate blocking the synthesis of aromatic amino acids. In those selected resistant mutants, more extended days of green leaves were observed after the glyphosate treatment, which allows economic and environmental savings and provides with more flexible management options.