Purification and Characterization of a Recombinant Caulobacter crescentus Epoxide Hydrolase
A Caulobacter crescentus epoxide hydrolase (CCEH) from a recombinant Escherichia coli was purified to homogeneity using a three-step procedure. The CCEH protein was purified 7.3-fold with a 22.9% yield in overall activity. The optimal reaction temperature and pH were determined to be 37℃ and pH 8.0,...
Saved in:
| Published in | Biotechnology and bioprocess engineering Vol. 11; no. 4; pp. 282 - 287 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
한국생물공학회
01.08.2006
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 1226-8372 1976-3816 |
| DOI | 10.1007/BF03026241 |
Cover
| Abstract | A Caulobacter crescentus epoxide hydrolase (CCEH) from a recombinant Escherichia coli was purified to homogeneity using a three-step procedure. The CCEH protein was purified 7.3-fold with a 22.9% yield in overall activity. The optimal reaction temperature and pH were determined to be 37℃ and pH 8.0, respectively. The addition of 10% (v/v) dimethylsulfoxide as a cosolvent improved the enantioselectivity of CCEH for a batch kinetic resolution of racemic indene oxide. |
|---|---|
| AbstractList | A Caulobacter crescentus epoxide hydrolase (CCEH) from a recombinant Escherichia coli was purified to homogeneity using a three-step procedure. The CCEH protein was purified 7.3-fold with a 22.9% yield in overall activity. The optimal reaction temperature and pH were determined to be 37℃ and pH 8.0, respectively. The addition of 10% (v/v) dimethylsulfoxide as a cosolvent improved the enantioselectivity of CCEH for a batch kinetic resolution of racemic indene oxide. A Caulobacter crescentus epoxide hydrolase (CCEH) from a recombinant Escherichia coli was purified to homogeneity using a three-step procedure. The CCEH protein was purified 7.3-fold with a 22.9% yield in overall activity. The optimal reaction temperature and pH were determined to be 37oC and pH 8.0, respectively. The addition of 10% (v/v) dimethylsulfoxide as a cosolvent improved the enantioselectivity of CCEH for a batch kinetic resolution of racemic indene oxide. KCI Citation Count: 10 |
| Author | Hwang, Seungha (Pohang University of Science and Technology, Pohang, Republic of Korea) Park, Y.S. (Pohang University of Science and Technology, Pohang, Republic of Korea) Choi, C.Y. (Seoul National University, Seoul, Republic of Korea), E-mail: choicy@snu.ac.kr Lee, E.Y. (Kyungsung University, Busan, Republic of Korea) Hyun, H.J. (Seoul National University, Seoul, Republic of Korea) Lee, B.J. (Seoul National University, Seoul, Republic of Korea) |
| Author_xml | – sequence: 1 fullname: Hwang, Seungha (Pohang University of Science and Technology, Pohang, Republic of Korea) – sequence: 2 fullname: Hyun, H.J. (Seoul National University, Seoul, Republic of Korea) – sequence: 3 fullname: Lee, B.J. (Seoul National University, Seoul, Republic of Korea) – sequence: 4 fullname: Park, Y.S. (Pohang University of Science and Technology, Pohang, Republic of Korea) – sequence: 5 fullname: Lee, E.Y. (Kyungsung University, Busan, Republic of Korea) – sequence: 6 fullname: Choi, C.Y. (Seoul National University, Seoul, Republic of Korea), E-mail: choicy@snu.ac.kr |
| BackLink | https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001211992$$DAccess content in National Research Foundation of Korea (NRF) |
| BookMark | eNotkEFLAzEUhINUsFYv3oUcvazmJbtJ9liX1hYLSqknD8vbbLbG1kSSLai_3tp6mmH4mIE5JwMfvCXkCtgtMKbu7qdMMC55DidkCKWSmdAgB3vPucy0UPyMnKf0zliutNZD8vq8i65zBnsXPEXf0uoNI5reRvdzDENHkS6tCR-N8-h7WuFuG5oDQ020yVjf7xKdfIYv11o6-25j2GKyF-S0w22yl_86Ii_TyaqaZYunh3k1XmQdB9ZnUliQncG8yIELVYDAtjWlMKWCvCwYAyPbRjDNjGokY2jbQgLTRoumLEGJEbk59vrY1Rvj6oDuoOtQb2I9Xq7mNSj11z4i10e0w1DjOrpUPy75_rn9iihB_ALEv1_K |
| ContentType | Journal Article |
| DBID | FBQ ACYCR |
| DOI | 10.1007/BF03026241 |
| DatabaseName | AGRIS Korean Citation Index |
| DatabaseTitleList | |
| Database_xml | – sequence: 1 dbid: FBQ name: AGRIS url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN sourceTypes: Publisher |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Engineering |
| EISSN | 1976-3816 |
| EndPage | 287 |
| ExternalDocumentID | oai_kci_go_kr_ARTI_177412 KR2007001391 |
| GroupedDBID | --- -4Y -58 -5G -BR -EM -Y2 -~C .86 .UV .VR 06C 06D 0R~ 0VY 1N0 203 23N 2J2 2JN 2JY 2KG 2KM 2LR 2VQ 2WC 2~H 30V 3V. 4.4 406 408 40D 40E 53G 5GY 5VS 6NX 7WY 7X7 85H 88A 88I 8AO 8FE 8FG 8FH 8FI 8FJ 8FL 8UJ 95- 95. 95~ 96X 9ZL A8Z AAAVM AABHQ AABYN AAFGU AAGCJ AAHNG AAIAL AAIKT AAJKR AANZL AARHV AARTL AATNV AATVU AAUCO AAUYE AAWCG AAYFA AAYIU AAYQN AAYTO ABDZT ABECU ABFGW ABFTV ABHQN ABJCF ABJNI ABJOX ABKAS ABKCH ABMNI ABMQK ABNWP ABQBU ABSXP ABTEG ABTHY ABTKH ABTMW ABUWG ABWNU ABXPI ACBMV ACBRV ACBXY ACBYP ACGFS ACGOD ACHSB ACHXU ACIGE ACIPQ ACIWK ACKNC ACMDZ ACMLO ACOKC ACOMO ACPRK ACSNA ACTTH ACVWB ACWMK ADBBV ADHHG ADHIR ADINQ ADKNI ADKPE ADMDM ADOXG ADRFC ADTPH ADURQ ADYFF ADZKW AEBTG AEEQQ AEFTE AEGAL AEGNC AEJHL AEJRE AEKMD AENEX AEOHA AEPYU AESKC AESTI AETLH AEVLU AEVTX AEXYK AFGCZ AFKRA AFLOW AFNRJ AFQWF AFRAH AFWTZ AFZKB AGAYW AGDGC AGGBP AGGDS AGJBK AGMZJ AGQMX AGWIL AGWZB AGYKE AHAVH AHBYD AHKAY AHMBA AHSBF AHYZX AIAKS AIIXL AILAN AIMYW AITGF AJBLW AJDOV AJGSW AJRNO AJZVZ AKQUC ALMA_UNASSIGNED_HOLDINGS ALWAN AMKLP AMXSW AMYLF AMYQR AOCGG ARAPS ARCSS ARMRJ ASPBG AVWKF AXYYD AYJHY AZFZN AZQEC B-. BA0 BBNVY BBWZM BDATZ BENPR BEZIV BGLVJ BGNMA BHPHI BPHCQ BVXVI CAG CCPQU COF CS3 CSCUP DDRTE DNIVK DPUIP DU5 DWQXO E3Z EBD EBLON EBS EIOEI EJD ESBYG ESTFP FBQ FEDTE FERAY FFXSO FIGPU FINBP FNLPD FRNLG FRRFC FSGXE FWDCC FYUFA G-Y G-Z GGCAI GGRSB GJIRD GNUQQ GNWQR GQ6 GQ7 GROUPED_ABI_INFORM_COMPLETE HCIFZ HF~ HG6 HMCUK HMJXF HRMNR HVGLF HZ~ I-F IJ- IKXTQ IWAJR IXC IXD I~X I~Z J-C J0Z JBSCW JZLTJ K60 K6~ KOV KVFHK L6V LK8 LLZTM M0C M0L M2P M4Y M7P M7S MA- ML0 MZR N2Q NDZJH NF0 NPVJJ NQJWS NU0 O9- O93 O9G O9I O9J OK1 P19 P2P P62 P9N PF0 PQBIZ PQQKQ PROAC PT4 PT5 PTHSS Q2X QOK QOR QOS R89 R9I RHV RNI RNS ROL RPX RSV RZK S16 S1Z S26 S27 S28 S3B SAP SCG SCLPG SCM SDH SHX SISQX SNE SNPRN SNX SOHCF SOJ SPISZ SQXTU SRMVM SSLCW STPWE SZN T13 T16 TSG TUC TUS U2A UG4 UKHRP UNUBA UOJIU UTJUX UZXMN VC2 VFIZW W48 W4F WK8 YLTOR Z45 Z5O Z7U Z7V Z7W Z8Q ZMTXR ZZE ~A9 AAPBV ACYCR |
| ID | FETCH-LOGICAL-f210t-63e16fca4541237513addc93c971495001c6db3080c7b600aed56108c83b99173 |
| ISSN | 1226-8372 |
| IngestDate | Tue Nov 21 21:24:57 EST 2023 Wed Dec 27 19:06:03 EST 2023 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 4 |
| Language | English |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-f210t-63e16fca4541237513addc93c971495001c6db3080c7b600aed56108c83b99173 |
| Notes | E21 2007001391 G704-000785.2006.11.4.001 |
| PageCount | 6 |
| ParticipantIDs | nrf_kci_oai_kci_go_kr_ARTI_177412 fao_agris_KR2007001391 |
| PublicationCentury | 2000 |
| PublicationDate | 2006-08-00 |
| PublicationDateYYYYMMDD | 2006-08-01 |
| PublicationDate_xml | – month: 08 year: 2006 text: 2006-08-00 |
| PublicationDecade | 2000 |
| PublicationTitle | Biotechnology and bioprocess engineering |
| PublicationYear | 2006 |
| Publisher | 한국생물공학회 |
| Publisher_xml | – name: 한국생물공학회 |
| SSID | ssj0047888 |
| Score | 1.7531432 |
| Snippet | A Caulobacter crescentus epoxide hydrolase (CCEH) from a recombinant Escherichia coli was purified to homogeneity using a three-step procedure. The CCEH... |
| SourceID | nrf fao |
| SourceType | Open Website Publisher |
| StartPage | 282 |
| SubjectTerms | Caulobacter crescentus chiral indene oxide chiral styrene oxide COMPOSE EPOXY COMPUESTOS EPOXY enzyme purification epoxide hydrolase EPOXY COMPOUNDS 생물공학 |
| Title | Purification and Characterization of a Recombinant Caulobacter crescentus Epoxide Hydrolase |
| URI | https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001211992 |
| Volume | 11 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| ispartofPNX | Biotechnology and Bioprocess Engineering, 2006, 11(4), , pp.282-287 |
| journalDatabaseRights | – providerCode: PRVLSH databaseName: SpringerLink Journals customDbUrl: mediaType: online eissn: 1976-3816 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0047888 issn: 1226-8372 databaseCode: AFBBN dateStart: 19961201 isFulltext: true providerName: Library Specific Holdings – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 1976-3816 dateEnd: 20241102 omitProxy: true ssIdentifier: ssj0047888 issn: 1226-8372 databaseCode: 7X7 dateStart: 19970601 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: http://www.proquest.com/pqcentral?accountid=15518 eissn: 1976-3816 dateEnd: 20241102 omitProxy: true ssIdentifier: ssj0047888 issn: 1226-8372 databaseCode: BENPR dateStart: 19970601 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Technology Collection customDbUrl: eissn: 1976-3816 dateEnd: 20241102 omitProxy: true ssIdentifier: ssj0047888 issn: 1226-8372 databaseCode: 8FG dateStart: 19970601 isFulltext: true titleUrlDefault: https://search.proquest.com/technologycollection1 providerName: ProQuest – providerCode: PRVAVX databaseName: SpringerLINK - Czech Republic Consortium customDbUrl: eissn: 1976-3816 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0047888 issn: 1226-8372 databaseCode: AGYKE dateStart: 19970101 isFulltext: true titleUrlDefault: http://link.springer.com providerName: Springer Nature – providerCode: PRVAVX databaseName: SpringerLink Journals (ICM) customDbUrl: eissn: 1976-3816 dateEnd: 99991231 omitProxy: true ssIdentifier: ssj0047888 issn: 1226-8372 databaseCode: U2A dateStart: 19970101 isFulltext: true titleUrlDefault: http://www.springerlink.com/journals/ providerName: Springer Nature |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3da9swEBchfdkexr7Kui80mJ6Ch23JtvQoOynZBmWMFgp7MJJsl1AaFzeBrf_1_oOdZCdRug26vQghny-S79D97nI6IfTeCJaZJuKgvADfGICGQKmGBWDOFVOJgrdcguxJOj9jn86T89Hop5e1tF7pD-b2j-dK_keqMAZytadk_0GyW6YwAH2QL7QgYWjvJeMv685m-vQytBHwYlt--XYLBZWFhu2Vdikvk0KtbQkQSzMBwOhqOa1vJrPr9vuiqifzH1UHzu7NXoJQvmhX2wi8-x29aK_7EwaTelfQcCM8MpsSIYgUZFYQLkku3EhCBN-RFCSHgcJ1QiK57QgJL-1x4YSzodPTyoxw5nMRU5IDSU5ySkTkSCgZ7uv2ohncj2ZspgMcpZ2GnLqZUssMOEmYUv-I2qcDcb8ISXjhbeQAKwNwvvudvu7HAHoF9o9Sf8PmsWf74974_2ZWwl2yfJzGfaWu_drdd2zqXvXuS7MoL9rysivBR_lYRoC47b3YBzEYonCMDuRxnp9s4IO9zsCd39wsYL-m7jABQEKNaqFddo2Hh04fo0eDI4Nlr5VP0KhePkUPvfKWz9A3Xz8x6A2-q5-4bbDCnn5iTz_xTj_xoJ94q5_P0dnx7LSYB8NlHkETR-EqSGkdpY1RLIHF0yyJKFhWI6gRmXXSAS2ZtNIUHBiTaUDhqq4stOeGUw0-TEYP0XjZLusXCBtNdcgaXaeVYIkQqgqpqSKlWRSZKhNH6BA-TqkuwEyWn7_aaLzzdKIj9A6-l5PHX-Xy8h40r9CDne6-RuNVt67fADZd6beDNH8BcQ19qg |
| linkProvider | Library Specific Holdings |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Purification+and+Characterization+of+a+Recombinant+Caulobacter+crescentus+Epoxide+Hydrolase&rft.jtitle=Biotechnology+and+bioprocess+engineering&rft.au=%ED%99%A9%EC%8A%B9%ED%95%98&rft.au=%EC%B5%9C%EC%B0%A8%EC%9A%A9&rft.au=%ED%98%84%ED%98%9C%EC%A7%84&rft.au=%EC%9D%B4%EB%B3%91%EC%A3%BC&rft.date=2006-08-01&rft.pub=%ED%95%9C%EA%B5%AD%EC%83%9D%EB%AC%BC%EA%B3%B5%ED%95%99%ED%9A%8C&rft.issn=1226-8372&rft.eissn=1976-3816&rft.spage=282&rft.epage=287&rft_id=info:doi/10.1007%2FBF03026241&rft.externalDBID=n%2Fa&rft.externalDocID=oai_kci_go_kr_ARTI_177412 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1226-8372&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1226-8372&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1226-8372&client=summon |