Purification and Characterization of a Recombinant Caulobacter crescentus Epoxide Hydrolase

• Published in: Biotechnology and bioprocess engineering Vol. 11; no. 4; pp. 282 - 287
• Main Authors: Hwang, Seungha (Pohang University of Science and Technology, Pohang, Republic of Korea), Hyun, H.J. (Seoul National University, Seoul, Republic of Korea), Lee, B.J. (Seoul National University, Seoul, Republic of Korea), Park, Y.S. (Pohang University of Science and Technology, Pohang, Republic of Korea), Lee, E.Y. (Kyungsung University, Busan, Republic of Korea), Choi, C.Y. (Seoul National University, Seoul, Republic of Korea), E-mail: choicy@snu.ac.kr
• Format: Journal Article
• Language: English
• Published: 한국생물공학회 01.08.2006
• Subjects: Caulobacter crescentus; chiral indene oxide; chiral styrene oxide; COMPOSE EPOXY; COMPUESTOS EPOXY; enzyme purification; epoxide hydrolase; EPOXY COMPOUNDS; 생물공학
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/BF03026241

A Caulobacter crescentus epoxide hydrolase (CCEH) from a recombinant Escherichia coli was purified to homogeneity using a three-step procedure. The CCEH protein was purified 7.3-fold with a 22.9% yield in overall activity. The optimal reaction temperature and pH were determined to be 37℃ and pH 8.0, respectively. The addition of 10% (v/v) dimethylsulfoxide as a cosolvent improved the enantioselectivity of CCEH for a batch kinetic resolution of racemic indene oxide.