The biosynthesis of nicotinamide adenine dinucleotides in bacteria

• Published in: Cofactor Biosynthesis: a Mechanistic Perspective Vol. 61; pp. 103 - 119
• Main Authors: Begley, Tadhg P, Kinsland, Cynthia, Mehl, Ryan A, Osterman, Andrei, Dorrestein, Pieter
• Format: Book Chapter Journal Article
• Language: English
• Published: United States Elsevier Science & Technology 2001; Academic Press
• Subjects: Amide Synthases - metabolism; Animal physiology; bacteria; Bacteriology; biochemical pathways; Biochemistry; Biological and medical sciences; biosynthesis; Endocrinology; enzymes; Escherichia coli - enzymology; eukaryotic cells; Fundamental and applied biological sciences. Psychology; humans; Kinetics; Metabolism. Enzymes; Microbiology; NAD (coenzyme); NAD - biosynthesis; NAD - metabolism; NADP - metabolism; nutrition physiology; Oxidation-Reduction; Phosphotransferases - metabolism; NAD; Eucaryote; Regulation(control); Enzyme; Metabolic cycle; Bacteria; Biosynthesis; Review; Cofactor
• ISBN: 9780127098616; 0127098615
• ISSN: 0083-6729; 2162-2620
• DOI: 10.1016/S0083-6729(01)61003-3

The nicotinamide adenine dinucleotides (NAD, NADH, NADP, and NADPH) are essential cofactors in all living systems and function as hydride acceptors (NAD, NADP) and hydride donors (NADH, NADPH) in biochemical redox reactions. The six-step bacterial biosynthetic pathway begins with the oxidation of aspartate to iminosuccinic acid, which is then condensed with dihydroxyacetone phosphate to give quinolinic acid. Phosphoribosylation and decarboxylation of quinolinic acid gives nicotinic acid mononucleotide. Adenylation of this mononucleotide followed by amide formation completes the biosynthesis of NAD. An additional phosphorylation gives NADP. This review focuses on the mechanistic enzymology of this Dathwav in bacteria.