Mixed function oxidase induction in Carcinus aestuarii. : Field and experimental studies for the evaluation of toxicological risk due to Mediterranean contaminants

• Published in: Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology Vol. 121; no. 1; pp. 321 - 331
• Main Authors: Fossi, M.C, Savelli, C, Casini, S
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.1998
• Subjects: Animals; B( a)P; Benzopyrene Hydroxylase - biosynthesis; BPH; Brachyura - enzymology; Carcinus aestuarii; Crustaceans; Cytochrome P-450; Cytochrome P-450 CYP1A1 - biosynthesis; Environmental Monitoring; Enzyme Induction; EROD; Evaluation Studies as Topic; Mediterranean Sea; Orbetello Lagoon; Venice Lagoon; Water Pollutants, Chemical - toxicity; Mediterranean Sea; MED, Italy, Grosetto, Orbetello Lagoon; MED, Italy, Venezia, Veneta Lagoon; Crustaceans; EROD; Orbetello Lagoon; Cytochrome P-450; Carcinus aestuarii; BPH; Venice Lagoon; B( a)P
• ISSN: 0742-8413; 1367-8280
• DOI: 10.1016/S0742-8413(98)10053-1

The aim of this study was to test and validate the use of mixed function oxidase (MFO) induction, in the crab Carcinus aestuarii, under experimental and field studies, for the evaluation of toxicological risk due to the main contaminants in the Mediterranean. Two different experiments were performed in the laboratory in order to identify the most suitable tissues for MFO studies in this species and the most suitable and sensitive MFO responses for evaluating chemical stress due to lipophilic contaminants. In order to validate this methodology in the field, two studies were carried out in two polluted Mediterranean lagoons: a transplant experiment in Orbetello Lagoon and an in situ experiment in Venice Lagoon. The following MFO responses were investigated in hepatopancres and gills of the crabs: ethoxyresorufin- O-deethylase (EROD) and benzo( a)pyrene hydroxylase (BPH) activities and reductase enzyme activities. The main results can be summarised as follows: midgut-gland and gills were confirmed to be useful for MFO tests; BPH activity in hepatopancreas was the most suitable and sensitive MFO response for evaluating chemical stress due to Mediterranean contaminants in laboratory and field studies; in the Orbetello Lagoon experiment, a statistically significant difference was found between sites subject to different human impact.