Utilization of PFGE as a Powerful Discriminative Tool for the Investigation of Genetic Diversity among MRSA Strains

Methicillin-resistant (MRSA) is a significant challenge to the burn patient. The implementation of proper monitoring programs and prompt identification of epidemic MRSA strains are critical to consequently control and eradicate potential outbreaks. This study aimed to define the genetic relatedness...

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Bibliographic Details
Published inIranian journal of public health Vol. 46; no. 3; pp. 351 - 356
Main Authors Ohadian Moghadam, Solmaz, Pourmand, Mohammad Reza, Douraghi, Masoumeh, Sabzi, Samira, Ghaffari, Parisa
Format Journal Article
LanguageEnglish
Published Iran Tehran University of Medical Sciences 01.03.2017
Subjects
Antibiotics
Bacteria
Burn units
Cloning
Colleges & universities
Deoxyribonucleic acid
Disease control
DNA
Drug resistance
Ethics
Genetic diversity
Laboratories
Methicillin-resistant staphylococcus aureus
Molecular typing
Nosocomial infections
Original
Pulsed-field gel electrophoresis
Staphylococcus infections
Iran
Methicillin-resistant staphylococcus aureus
Molecular typing
Pulsed-field gel electrophoresis
Burn units
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ISSN2251-6085
2251-6093

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Summary:Methicillin-resistant (MRSA) is a significant challenge to the burn patient. The implementation of proper monitoring programs and prompt identification of epidemic MRSA strains are critical to consequently control and eradicate potential outbreaks. This study aimed to define the genetic relatedness of MRSA strains isolated from burn patients by analyzing the large fragments of DNA. In this cross-sectional study, 126 pus/wound swabs from skin and soft tissue infections (SSTIs) were collected from inpatients of Shahid Motahari Burn Center (Tehran, Iran) in 2013. Then, molecular typing of MRSA was achieved by Pulsed-Field Gel Electrophoresis (PFGE). The PFGE analysis of MRSA indicated 31 single types and 5 common types. There was a significant diversity in the chromosomal digestion pattern of the MRSA strains explained by the acquisition of MRSA from various sources. The permanent import of novel types of MRSA strains despite the rigorous infection control measures carried out within the center. The importance of PFGE in understanding the epidemiology of MRSA may serve as a basis for the development of rational control strategies.
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ISSN:2251-6085
2251-6093