PHF5A Promotes Proliferation and Migration of Non-Small Cell Lung Cancer 
by Regulating of PI3K/AKT Pathway

• Published in: Zhongguo fei ai za zhi Vol. 26; no. 1; pp. 10 - 16
• Main Authors: Wang, Houhui, Liu, Fanglei, Bai, Chunxue, Xu, Nuo
• Format: Journal Article
• Language: Chinese
• Published: China Chinese Anti-Cancer Association Chinese Antituberculosis Association 2023; Chinese Anti-Cancer Association; Chinese Antituberculosis Association
• Subjects: Brain cancer; Carcinoma, Non-Small-Cell Lung - pathology; Cell cycle; Cell growth; Cell Line, Tumor; Cell Movement - genetics; Cell Proliferation - genetics; Gene Expression Regulation, Neoplastic; Humans; Lung cancer; lung neoplasms; Lung Neoplasms - pathology; migration; phf5a; Phosphatidylinositol 3-Kinases - genetics; Phosphatidylinositol 3-Kinases - metabolism; proliferation; Proteins; Proto-Oncogene Proteins c-akt - genetics; Proto-Oncogene Proteins c-akt - metabolism; RNA polymerase; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Trans-Activators - genetics; Wound healing; China; PHF5A; Proliferation; Migration; Lung neoplasms
• ISSN: 1009-3419; 1999-6187; 1999-6187
• DOI: 10.3779/j.issn.1009-3419.2023.102.01

There have been many significant advances in the diagnosis and treatment of non-small cell lung cancer (NSCLC). However, the mechanism underlying the progression of NSCLC is still not clear. Plant homodomain finger-like domain-containing protein 5A (PHF5A) plays an important role in processes of chromatin remodeling, morphological development of tissues and organs and maintenance of stem cell pluripotency. This study aims to investigate the role of PHF5A in the proliferation and migration of NSCLC. A549 and PC-9 PHF5A overexpression cell lines were constructed. PHF5A expression was decreased in H292 and H1299 cells by using siRNA. Flow cytometry was used to detect the cell cycle. MTT assay and clone formation assay were used to examine the proliferative ability of NSCLC, while migration assay and wound healing assay were performed to evaluate the ability of migration. Western blot analysis was used to measure the expressions of PI3K, p-AKT and the associated downstream factors. Up-regulation of PHF5A in A549