Diversity of Helicobacter pylori isolates expressing the major protein antigens and inducing specific antibodies in host

• Published in: Zhejiang da xue xue bao. Journal of Zhejiang University. Sciences edition. Li xue ban Vol. 36; no. 1; pp. 90 - 96
• Main Authors: Zhu, Liam, Tang, Ren-Xian, Shao, Jian-Zhong
• Format: Journal Article
• Language: Chinese
• Published: Zhejiang University Press 01.01.2009
• Subjects: 主要蛋白抗原; 幽门螺杆菌; 抗体水平; 表达频率
• ISSN: 1008-9497
• DOI: 10.3785/j.issn.1008-9497.2009.01.019

ELISAs were established based on recombinant protein antigens rUreB, rHpaA, rVacA, rCagAl, rNapA, rFlaA and rFlaB of Helocobacter pylori to detect the expression rates of the antigens in the bacterial isolates as well as the positive rates of the antigenic antibodies in the sera from H. pylori-infected patients. And meanwhile PCR was applied to detect the carrying rates of the genes encoding the antigens in the isolates. All the research works is to obtain evidence for selection of antigens used in genetic engineering vaccine of Helicobacter pylori.