重离子诱发的2个水稻突变体表型鉴定及遗传分析

【目的】明确2个水稻突变体的表型特征与遗传方式。【方法】通过重离子诱变野生型籼稻种质BBS，从其M2代中筛选出2个突变体，分别命名为m2和m3。通过表型观察和性状比较，对突变体材料进行鉴定；构建了粳稻种质02428(父本)与m2、m3的F2群体，并进行遗传分析。【结果】与野生型BBS相比，m2全生育期叶宽极显著变窄且内卷；m2剑叶、倒2叶和倒3叶的卷曲度分别为22.30%、38.15%和28.84%，与野生型BBS差异达到极显著水平；m2表现出高度不育。早季播种后第54天、晚季播种后第30天，m3从主茎新叶叶梢开始枯萎，整个叶枯表型持续25d左右，之后新长出的叶片恢复正常；m3主穗质量和主穗粒...

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Published in华南农业大学学报 Vol. 39; no. 1; pp. 12 - 17
Main Author 彭歆;罗立新;张力;熊子墨;王慧;郭涛;陈志强;肖武名
Format Journal Article
LanguageChinese
Published 2018
Subjects
水稻;重离子诱变;突变体;窄叶;叶片枯萎
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ISSN1001-411X
DOI10.7671/j.issn.1001-411X.2018.01.003

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Summary:【目的】明确2个水稻突变体的表型特征与遗传方式。【方法】通过重离子诱变野生型籼稻种质BBS，从其M2代中筛选出2个突变体，分别命名为m2和m3。通过表型观察和性状比较，对突变体材料进行鉴定；构建了粳稻种质02428(父本)与m2、m3的F2群体，并进行遗传分析。【结果】与野生型BBS相比，m2全生育期叶宽极显著变窄且内卷；m2剑叶、倒2叶和倒3叶的卷曲度分别为22.30%、38.15%和28.84%，与野生型BBS差异达到极显著水平；m2表现出高度不育。早季播种后第54天、晚季播种后第30天，m3从主茎新叶叶梢开始枯萎，整个叶枯表型持续25d左右，之后新长出的叶片恢复正常；m3主穗质量和主穗粒数极显著下降，其他农艺性状与野生型BBS无显著差异。遗传分析结果表明，m2/02428的F2群体剑叶宽的频率分布符合正态分布，m3/02428的F2群体中正常个体与叶片枯萎个体的分离比符合3∶1的理论比值。【结论】m2为窄叶突变体，其窄叶性状受多个基因控制；m3为叶片枯萎突变体，其突变性状受1对隐性核基因控制。
Bibliography:rice (Oryza sativa L.); heavy ion irradiation; mutant; narrow leaf; leaf necrosis
Objective】To clarify the phenotypic characteristics and genetic patterns of two rice(Oryza sativa L.)mutants.【Method】Two mutants were screened from the M2generation of the indica rice germplasm BBS(wild type)induced by heavy ion,named m2and m3respectively.Firstly,the mutant materials were identified by means of phenotypic observation and agronomic trait comparison.Secondly,the F2populations of02428(male parent)/m2and02428/m3were constructed for genetic analysis.【Result】Compared with BBS,the leaves of m2were inner-rolled and the leaf width was significantly reduced during the whole growth period.The rolling index of flag leaf,the2nd and3rd leaf from the top were22.30%,38.15%and28.84%respectively,which were significantly different from BBS.The mutant m2showed a high level of sterility.The new leaf tip of m3main stem started necrosis on the54th day after sowing in early season or30th day after sowing in late season.The leaf necrosis
ISSN:1001-411X
DOI:10.7671/j.issn.1001-411X.2018.01.003