蓝鹇源H9N2亚型禽流感病毒HA和NA基因序列分析

为了探查禽流感病毒H9N2在圈养蓝鹇中的流行规律及变异情况,从圈养蓝鹇（Lophura swinhoii）分离1株H9N2亚型禽流感病毒,对其HA及NA基因进行PCR扩增并进行序列分析。本分离株的HA基因序列与Genbank中广西鸡源2014年分离株A/chicken/Guangxi/CLB02/2014（H9N2）（KT023046）的核苷酸序列相似性最高,为99%;而NA基因与登录号为KM455874,2013人源分离株A/Lengshuitan/11197/2013（H9N2）的NA核苷酸序列相似性最高,为97%。HA蛋白序列分析显示：HA的226位氨基酸残基为亮氨酸（Leu）,具有哺乳...

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Published in野生动物学报 Vol. 38; no. 3; pp. 441 - 446
Main Author 单芬 王念晨 王文清 焦培荣 陈武 李婉萍 彭仕明
Format Journal Article
LanguageChinese
Published Editorial Department of Chinese Journal of Wildlife 2017
Subjects
H9N2亚型
序列分析
禽流感病毒
进化树
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ISSN2310-1490

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Summary:为了探查禽流感病毒H9N2在圈养蓝鹇中的流行规律及变异情况,从圈养蓝鹇（Lophura swinhoii）分离1株H9N2亚型禽流感病毒,对其HA及NA基因进行PCR扩增并进行序列分析。本分离株的HA基因序列与Genbank中广西鸡源2014年分离株A/chicken/Guangxi/CLB02/2014（H9N2）（KT023046）的核苷酸序列相似性最高,为99%;而NA基因与登录号为KM455874,2013人源分离株A/Lengshuitan/11197/2013（H9N2）的NA核苷酸序列相似性最高,为97%。HA蛋白序列分析显示：HA的226位氨基酸残基为亮氨酸（Leu）,具有哺乳动物SAα2-6受体结合的特性,裂解位点处的基序PSRSSR↓GL,明显发生1个碱性氨基酸的变异。HA蛋白具有9个潜在N-糖基化位点,分别位于29、82、141、218、298、305、313、492、551位;HA受体结合位点氨基酸变异明显。NA蛋白序列分析显示：NA蛋白颈部63~65位氨基酸缺失,具有7个潜在的糖基化位点,分别位于69、86、146、200、234、264、368位;NA蛋白红细胞结合位点、抗原决定簇区域氨基酸变异活跃。HA、NA基因进化树分析显示该分离株属于欧亚分支中Y280-Like小进化分支,与疫苗株虽属于同一进化分支中,但亲缘关系较远。蓝鹇源H9N2亚型禽流感病毒（LS/GD/P29/16）HA和NA基因发生一定程度的变异,需要加强流行病学监测。
Bibliography:Avian influenza vires;H9N2 subtype;Sequence analysis;Evolutionary tree
We studied genetic mutations of the genes for neuraminidase and hemagglutinin and the molecular epidemiology of H9N2 subtype Avian Influenza Virus（ AIV） in captive swinhoe＇s pheasant （ Lophura swinhoii）. H9N2 AIV strain was isolated from captive swinhoe＇s pheasant in Guangdong province. The entire NA and HA gene fragments of the strain were amplified by PCR and sequences were analyzed. The similarity of the strain HA gene was 99% compared with H9N2 AIV A/chicken/Guangxi/CLB02/2014（ H9N2 ） （KT023046）which was the most similar sequence in Genbank. The similarity of the strain NA gene was 97% compared with H9N2 AIV A/Lengshuitan/ 11197/2013（H9N2） （KM455874）which was the most similar sequence in Genbank. The sequence analysis of the strain HA protein showed that the amino residue at site 226 was leucine. It had the characteristics of the mammal SAa2 -6 receptor binding, namely one basic amino had obvious variation in the cleavage site motif PSR
ISSN:2310-1490