HPLC法测定蓝狐胆汁酸成分与含量

建立测定蓝狐（Vulpes lagopus）胆汁酸成分与含量的高效液相色谱方法。其方法为：采用HPLC-UV法,色谱柱为Waters C_（18）,流动相为甲醇和KH_2PO_4溶液,KH_2PO_4溶液浓度为0.25~0.35 mol/L（pH=3~4）,等度洗脱,流速为1.1 mL/min,检测波长为205 nm。结果：蓝狐胆囊胆汁中含牛磺熊去氧胆酸（TUDCA）、牛磺胆酸（TCA）、牛磺鹅去氧胆酸（TCDCA）和牛磺去氧胆酸（TDCA）,分别约占蓝狐胆汁质量的0.010 1%,0.171 8%,0.039 6%和0.079 9%。结论：胆汁酸各组分的线性关系良好,本方法准确可靠,重复性好...

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Published in野生动物学报 Vol. 37; no. 2; pp. 108 - 112
Main Author 黄金野 张晶钰 柴龙会 刘菁菁 肖向红
Format Journal Article
LanguageChinese
Published Editorial Department of Chinese Journal of Wildlife 2016
Subjects
含量测定
狐胆汁
胆汁酸
高效液相色谱
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ISSN2310-1490

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Summary:建立测定蓝狐（Vulpes lagopus）胆汁酸成分与含量的高效液相色谱方法。其方法为：采用HPLC-UV法,色谱柱为Waters C_（18）,流动相为甲醇和KH_2PO_4溶液,KH_2PO_4溶液浓度为0.25~0.35 mol/L（pH=3~4）,等度洗脱,流速为1.1 mL/min,检测波长为205 nm。结果：蓝狐胆囊胆汁中含牛磺熊去氧胆酸（TUDCA）、牛磺胆酸（TCA）、牛磺鹅去氧胆酸（TCDCA）和牛磺去氧胆酸（TDCA）,分别约占蓝狐胆汁质量的0.010 1%,0.171 8%,0.039 6%和0.079 9%。结论：胆汁酸各组分的线性关系良好,本方法准确可靠,重复性好,可用于测定蓝狐胆汁酸成分及含量,对合理开发和利用蓝狐胆汁资源具有积极意义。
Bibliography:Huang Jinye,Zhang Jingyu,Chai Longhui,Liu Jingjing,Xiao Xianghong （Northeast Forestry University, Harbin, 150040, China）
HPLC（High-performance liquid chromatography）; Fox bile; Bile acid; Content
We developed a method to assess the bile acid composition and content of blue fox（Vulpes lagopus） by high- performance liquid chromatography（HPLC） with ultra- violet（HPLC-UV）.We used Waters C_（18） and the mobile phase was methanol and KH_2PO_4,concentration of KH_2PO_4 solution was 0.25-0.35 mol/L（pH =3-4）,isocratic elution.The flow rate was1.1 mL/min and the detection wavelength was 205 nm.Blue fox bile contained TUDCA,TCA,TCDCA and TDCA,accounting for 0.0101%,0.1718%,0.0396%and 0.0799%of blue fox gallbladder bile quality,respectively.Each component of blue fox bile showed good linear relation.This analytical method proved reliable,accurate,and repeatable,and it can be used to assay the bile acid composition and content of blue fox.These results are important for the development and utilization of blue fox bile.
23-158
ISSN:2310-1490