LIPID PEROXIDATION AND ANTIOXIDANT DEFENSE INDICES IN LIVER TISSUE OF RATS FOLLOWING EXPERIMENTAL SURGICAL TRAUMA AND POST-TRAUMATIC STRESS DISORDER

• Published in: Актуальні проблеми сучасної медицини: Вісник Української медичної стоматологічної академії Vol. 25; no. 2; pp. 160 - 163
• Main Authors: Riabushko, R.M., Kostenko, V.O.
• Format: Journal Article
• Language: English
• Published: 29.05.2025
• ISSN: 2077-1096; 2077-1126
• DOI: 10.31718/2077-1096.25.2.166

This study investigated changes in the concentration of secondary lipid peroxidation (LPO) products and the state of antioxidant defense in liver tissue of rats exposed to surgical trauma reproduced under experimental post-traumatic stress disorder (PTSD). The series of experiments were conducted on 42 male Wistar rats weighing 210–230 g, divided into six groups: Group 1 included intact animals; Group 2 involved animals subjected to a single prolonged stress (SPS) model of PTSD; Group 3 included sham-operated animals; Group 4 consisted of animals subjected to laparotomy; Group 5 involved animals exposed to SPS followed by sham surgery; Group 6 included animals subjected to laparotomy under modeled PTSD. The concentration of secondary LPO products in liver homogenates was measured spectrophotometrically. The findings showed that SPS induced activation of LPO processes in the liver, manifested by an increase in secondary LPO products and a decrease in antioxidant defense efficiency. Surgical trauma in the form of laparotomy led to a significant enhancement of free radical oxidation in liver tissue; however, these changes were less pronounced compared to the group exposed to the combined effects of surgical and psychoemotional stress. The combination of surgical trauma and PTSD resulted in the highest levels of LPO in liver tissue, indicating a synergistic effect of both pathogenic factors on cellular damage and depletion of antioxidant protection. These findings highlight the need for further research aimed at developing therapeutic strategies focused on redox regulation and the prevention of metabolic complications associated with the combined effects of PTSD and surgical stress.