A Combined Transcriptomics and Lipidomics Analysis of Subcutaneous, Epididymal and Mesenteric Adipose Tissue Reveals Marked Functional Differences

• Published in: PloS one Vol. 5; no. 7; p. e11525
• Main Authors: Caesar, R, Manieri, M, Kelder, T, Boekschoten, M.V, Evelo, C, Müller, M.R, Kooistra, T, Cinti, S, Kleemann, R, Drevon, C.A
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 12.07.2010; Public Library of Science (PLoS)
• Subjects: Adipocytes; Adipocytes - metabolism; Adipose tissue; Adipose Tissue - metabolism; Albinism; Androgen receptors; Animal tissues; Animals; Bioinformatics; Body fat; Carbohydrates; Consortia; Desaturation; Diabetes and Endocrinology/Endocrinology; Diabetes and Endocrinology/Obesity; Diet; DNA microarrays; element-binding protein; Elongation; Epididymis; Epididymis - metabolism; Fatty acids; Fatty Acids, Omega-3 - metabolism; Fatty Acids, Omega-6 - metabolism; Fatty Acids, Unsaturated - metabolism; fatty-acid-composition; Gene expression; Gene Expression Profiling - methods; Genetic engineering; Genomics; glucose-metabolism; High fat diet; Histology; Hormones; human preadipocytes; in-vitro; insulin sensitivity; Kinases; Laboratory animals; Linoleic acid; Linolenic acid; Linolenic acids; Lipogenesis; Lipogenesis - genetics; Lipogenesis - physiology; lymphoid-cells; Male; Medical and Health Sciences; Medical research; Medicin och hälsovetenskap; Medicine; Mesentery - metabolism; messenger-rna expression; Metabolic pathways; Metabolic syndrome; Mice; Mice, Transgenic; Nuclear Proteins; Nutrition research; obese subjects; Obesity; Oligonucleotide Array Sequence Analysis; Omega 3 fatty acids; Phosphorylation; Physiology; Physiology/Genomics; Polyunsaturated fatty acids; Proteins; Quality of life; Reverse Transcriptase Polymerase Chain Reaction; Rodents; Sperm; Spermatogenesis; Subcutaneous Tissue - metabolism; Testosterone; Transcription Factors; Transgenic mice; Zonation; Norway; Netherlands
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0011525

Depot-dependent differences in adipose tissue physiology may reflect specialized functions and local interactions between adipocytes and surrounding tissues. We combined time-resolved microarray analyses of mesenteric- (MWAT), subcutaneous- (SWAT) and epididymal adipose tissue (EWAT) during high-fat feeding of male transgenic ApoE3Leiden mice with histology, targeted lipidomics and biochemical analyses of metabolic pathways to identify differentially regulated processes and site-specific functions. EWAT was found to exhibit physiological zonation. De novo lipogenesis in fat proximal to epididymis was stably low, whereas de novo lipogenesis distal to epididymis and at other locations was down-regulated in response to high-fat diet. The contents of linoleic acid and alpha-linolenic acid in EWAT were increased compared to other depots. Expression of the androgen receptor (Ar) was higher in EWAT than in MWAT and SWAT. We suggest that Ar may mediate depot-dependent differences in de novo lipogenesis rate and propose that accumulation of linoleic acid and alpha-linolenic acid in EWAT is favored by testosterone-mediated inhibition of de novo lipogenesis and may promote further elongation and desaturation of these polyunsaturated fatty acids during spermatogenesis.