骨髓间充质干细胞对冈田酸致神经细胞损伤的修复作用
目的 观察人骨髓间充质干细胞对冈田酸（OA）损伤的神经细胞是否具有修复作用。方法 应用冈田酸损伤神经母细胞瘤细胞系SH-SY5Y细胞,建立阿尔茨海默病体外模型。将细胞分成正常组、损伤组和治疗组,损伤组用20nmol/L冈田酸损伤24h,治疗组在损伤24h后加入骨髓间充质干细胞的条件培养基治疗24h。采用CCK-8法检测各组细胞活力,免疫荧光染色微管微丝测定细胞树突长度和荧光面积,Western blotting检测磷酸化Tau蛋白和总Tau蛋白含量。结果 冈田酸能损伤SH-SY5Y细胞,使其胞体皱缩、塌陷,出现空泡,树突缩短、断裂,微管微丝排列紊乱,而骨髓间充质干细胞条件培养基可使SH-SY5...
Saved in:
| Published in | Jie fang jun yi xue za zhi Vol. 42; no. 5; pp. 377 - 382 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
Beijing
People's Military Medical Press
2017
510515 广州 南方医科大学检验与生物技术学院 100850 北京 军事医学科学院野战输血研究所干细胞与再生医学研究室%100850,北京 军事医学科学院野战输血研究所干细胞与再生医学研究室 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0577-7402 |
| DOI | 10.11855/j.issn.0577-7402.2017.05.04 |
Cover
| Abstract | 目的 观察人骨髓间充质干细胞对冈田酸（OA）损伤的神经细胞是否具有修复作用。方法 应用冈田酸损伤神经母细胞瘤细胞系SH-SY5Y细胞,建立阿尔茨海默病体外模型。将细胞分成正常组、损伤组和治疗组,损伤组用20nmol/L冈田酸损伤24h,治疗组在损伤24h后加入骨髓间充质干细胞的条件培养基治疗24h。采用CCK-8法检测各组细胞活力,免疫荧光染色微管微丝测定细胞树突长度和荧光面积,Western blotting检测磷酸化Tau蛋白和总Tau蛋白含量。结果 冈田酸能损伤SH-SY5Y细胞,使其胞体皱缩、塌陷,出现空泡,树突缩短、断裂,微管微丝排列紊乱,而骨髓间充质干细胞条件培养基可使SH-SY5Y细胞胞体变得圆润,树突重新恢复变长,微管、微丝致密规则,荧光变强;并且能有效降低冈田酸诱导的Tau蛋白过度磷酸化水平。结论 骨髓间充质干细胞对冈田酸损伤的神经细胞具有明显的修复作用。 |
|---|---|
| AbstractList | R749.16; 目的 观察人骨髓间充质干细胞对冈田酸(OA)损伤的神经细胞是否具有修复作用.方法 应用冈田酸损伤神经母细胞瘤细胞系SH-SY5Y细胞,建立阿尔茨海默病体外模型.将细胞分成正常组、损伤组和治疗组,损伤组用20nmol/L冈田酸损伤24h,治疗组在损伤24h后加入骨髓间充质于细胞的条件培养基治疗24h.采用CCK-8法检测各组细胞活力,免疫荧光染色微管微丝测定细胞树突长度和荧光面积,Western blotting检测磷酸化Tau蛋白和总Tau蛋白含量.结果 冈田酸能损伤SH-SY5Y细胞,使其胞体皱缩、塌陷,出现空泡,树突缩短、断裂,微管微丝排列紊乱,而骨髓间充质干细胞条件培养基可使SH-SY5Y细胞胞体变得圆润,树突重新恢复变长,微管、微丝致密规则,荧光变强;并且能有效降低冈田酸诱导的Tau蛋白过度磷酸化水平.结论 骨髓间充质干细胞对冈田酸损伤的神经细胞具有明显的修复作用. Objective To study whether the human bone marrow mesenchymal stem cells (HBMSCs) can repair damaged neural cells induced by okadaic acid (OA). Methods Neuroblastoma cell line SH-SY5Y cells were used to incubate with 20nmol/L okadaic acid for 24h, establishing Alzheimer's Disease cell model; Three groups were set up: normal group, okadaic acid -damaged (OA-damaged) group, hBMSCs -treatment group. The cells were injured for 24h with 20nmol/L OA in OA-damaged group, and treated with conditioned medium obtaining hBMSCs for 24h after 24h OA injury in the treatment group. Then CCK-8 was used for detecting cell vitality, immune fluorescence dyed microtubules and microfilaments for determining the dendritic cell length and fluorescence intensity, in addition, Western blotting for analyzing the protein level of phosphorylated tau and total tau proteins. Results Okadaic acid damaged SH-SY5Y cells, contributed to shrinkage, collapse, cavitation of the SH-SY5Y cell body, dendritic shortening and fracture, and irregular a 目的 观察人骨髓间充质干细胞对冈田酸（OA）损伤的神经细胞是否具有修复作用。方法 应用冈田酸损伤神经母细胞瘤细胞系SH-SY5Y细胞,建立阿尔茨海默病体外模型。将细胞分成正常组、损伤组和治疗组,损伤组用20nmol/L冈田酸损伤24h,治疗组在损伤24h后加入骨髓间充质干细胞的条件培养基治疗24h。采用CCK-8法检测各组细胞活力,免疫荧光染色微管微丝测定细胞树突长度和荧光面积,Western blotting检测磷酸化Tau蛋白和总Tau蛋白含量。结果 冈田酸能损伤SH-SY5Y细胞,使其胞体皱缩、塌陷,出现空泡,树突缩短、断裂,微管微丝排列紊乱,而骨髓间充质干细胞条件培养基可使SH-SY5Y细胞胞体变得圆润,树突重新恢复变长,微管、微丝致密规则,荧光变强;并且能有效降低冈田酸诱导的Tau蛋白过度磷酸化水平。结论 骨髓间充质干细胞对冈田酸损伤的神经细胞具有明显的修复作用。 |
| Abstract_FL | Objective To study whether the human bone marrow mesenchymal stem cells (HBMSCs) can repair damaged neural cells induced by okadaic acid (OA).Methods Neuroblastoma cell line SH-SY5Y cells were used to incubate with 20nmol/L okadaic acid for 24h,establishing Alzheimer's Disease cell model;Three groups were set up:normal group,okadaic acid-damaged (OA-damaged) group,hBMSCs-treatment group.The cells were injured for 24h with 20nmol/L OA in OA-damaged group,and treated with conditioned medium obtaining hBMSCs for 24h after 24h OA injury in the treatment group.Then CCK-8 was used for detecting cell vitality,immune fluorescence dyed microtubules and micro filaments for determining the dendritic cell length and fluorescence intensity,in addition,Western blotting for analyzing the protein level of phosphorylated tau and total tau proteins.Results Okadaic acid damaged SH-SY5Y cells,contributed to shrinkage,collapse,cavitation of the SH-SY5Y cell body,dendritic shortening and fracture,and irregular arrangement of microtubule microfilaments;while BMSCs conditioned medium made SHSYSY cell body become round and longer,dendrites restored,and microtubules and microfilaments arranged regularly,fluorescence intensity enhanced.Meanwhile,it also down-regulated the level of OA-induced tau phosphorylation.Conclusion hBMSCs have repair effects on the neural cell damage induced by okadaic acid. |
| Author | 刘嘉婧 曹宁 翟晶磊 廖土玲 岳文 贾雅丽 裴雪涛 |
| AuthorAffiliation | 南方医科大学检验与生物技术学院,广州510515 军事医学科学院野战输血研究所干细胞与再生医学研究室,北京100850 |
| AuthorAffiliation_xml | – name: 510515 广州 南方医科大学检验与生物技术学院;100850 北京 军事医学科学院野战输血研究所干细胞与再生医学研究室%100850,北京 军事医学科学院野战输血研究所干细胞与再生医学研究室 |
| Author_FL | LIU Jia-jing LIAO Tu-ling JIA Ya-li ZHAI Jing-lei CAO Ning YUE Wen PEI Xue-tao |
| Author_FL_xml | – sequence: 1 fullname: LIU Jia-jing – sequence: 2 fullname: CAO Ning – sequence: 3 fullname: ZHAI Jing-lei – sequence: 4 fullname: LIAO Tu-ling – sequence: 5 fullname: YUE Wen – sequence: 6 fullname: JIA Ya-li – sequence: 7 fullname: PEI Xue-tao |
| Author_xml | – sequence: 1 fullname: 刘嘉婧 曹宁 翟晶磊 廖土玲 岳文 贾雅丽 裴雪涛 |
| BookMark | eNpFkMFKw0AURWdRwVr7E-rCTeLMZDJJllLUCgU33ZfJJFMTNGkTi7ZbbXVTXFgQROqmQlFQkS4sVfyZxjR_YaSiq8t793Af7y6BjOd7NgBrCMoI6aq64cpOGHoyVDVN0gjEMoZIS0cZkgzI_q0XQT4MHRNChCAhiGZBMXkYJo9XyfUoardno2E0fo0nndlpP3oeR52LuPeStN9m56P4vh9PLufWV_du-j6Ib86mn0_RoDv9uI17w2WwINhBaOd_NQfK21vlQlEq7e3sFjZLEtd1IqlcEMo5VxCnEOrc5AiqhmGahqJZyGTEJEI3bMypYWLdQphZzMYaZrZAliaEkgPr89hj5gnmVSuu3wi89GDFFW7zpNX6eRyqEJIUXZ2jtcCvN-zw6J_FaQOUqoqCUmplTvF936vWnTSyFjiHLGhWqIYxJDqlyjfTRYSp |
| ClassificationCodes | R749.16 |
| ContentType | Journal Article |
| Copyright | 2017] This work is licensed under [CC BY 3.0 Unported - https://creativecommons.org/licenses/by/3.0/ (“the License”). Notwithstanding the ProQuest Terms and conditions, you may use this content in accordance with the terms of the License. Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: 2017] This work is licensed under [CC BY 3.0 Unported - https://creativecommons.org/licenses/by/3.0/ (“the License”). Notwithstanding the ProQuest Terms and conditions, you may use this content in accordance with the terms of the License. – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP W91 ~WA 3V. 7XB 88F 8FK ABUWG AFKRA BENPR BVBZV CCPQU DWQXO M1Q PHGZM PHGZT PKEHL PQEST PQQKQ PQUKI PRINS Q9U 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.11855/j.issn.0577-7402.2017.05.04 |
| DatabaseName | 维普期刊资源整合服务平台 中文科技期刊数据库-CALIS站点 维普中文期刊数据库 中文科技期刊数据库-医药卫生 中文科技期刊数据库- 镜像站点 ProQuest Central (Corporate) ProQuest Central (purchase pre-March 2016) Military Database (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest Central UK/Ireland ProQuest Central East & South Asia Database ProQuest One Community College ProQuest Central Military Database Proquest Central Premium ProQuest One Academic ProQuest One Academic Middle East (New) ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China ProQuest Central Basic Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitle | ProQuest One Academic Middle East (New) ProQuest Central Basic ProQuest One Academic Eastern Edition ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest Military Collection (Alumni Edition) ProQuest Military Collection ProQuest Central China East & South Asia Database ProQuest Central ProQuest One Academic UKI Edition ProQuest Central Korea ProQuest Central (New) ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) |
| DatabaseTitleList | ProQuest One Academic Middle East (New) |
| Database_xml | – sequence: 1 dbid: BENPR name: ProQuest Central url: http://www.proquest.com/pqcentral?accountid=15518 sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| DocumentTitleAlternate | Role of bone marrow mesenchymal stem ceils in repair of neural cell injury induced by okadaic acid |
| DocumentTitle_FL | Role of bone marrow mesenchymal stem cells in repair of neural cell injury induced by okadaic acid |
| EndPage | 382 |
| ExternalDocumentID | jfjyxzz201705004 672204866 |
| GrantInformation_xml | – fundername: 国家高技术研究发展计划(863计划)项目; 广州市健康医疗协同创新重大专项; 广东省重大科技专项; 广东省前沿与关键技术创新专项(2014B020228001,2015B020226001)This work was supported by the National High Technology Research and Development Program (863 Program) of China; Guangzhou Health Care and Cooperative Innovation Major Project; Guangdong Major Scientific and Technological Project; Guangdong Frontier and Key Technology Innovation Project funderid: (2013AA020107); (201604020009); (2013A022100005); (2013AA020107); (201604020009); (2013A022100005); (2014B020228001,2015B020226001) |
| GroupedDBID | -05 2B. 2C~ 2RA 3V. 5VS 5XA 5XF 92F 92I 92L ABUWG ACGFS AFKRA ALMA_UNASSIGNED_HOLDINGS BENPR BPHCQ BVBZV CCEZO CCPQU CIEJG CQIGP CW9 GROUPED_DOAJ IPNFZ KQ8 PQQKQ PROAC RIG RNS TCJ TGQ U1G U5O W91 ~WA 7XB 8FK DWQXO M1Q PHGZM PHGZT PKEHL PQEST PQUKI PRINS Q9U 4A8 93N PSX |
| ID | FETCH-LOGICAL-c884-5cf46ccc31c6008cbc10599bb937d1ba4b4f89e2c69b28d12adae272aef1d7ff3 |
| IEDL.DBID | BENPR |
| ISSN | 0577-7402 |
| IngestDate | Thu May 29 04:00:06 EDT 2025 Mon Jun 30 07:37:26 EDT 2025 Wed Feb 14 10:01:49 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 5 |
| Keywords | 冈田酸 tau蛋白质类 tau proteins Alzheimer disease bone mesenchymal stem cells 骨髓间充质干细胞 阿尔茨海默病 okadaic acid |
| Language | Chinese |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c884-5cf46ccc31c6008cbc10599bb937d1ba4b4f89e2c69b28d12adae272aef1d7ff3 |
| Notes | Objective To study whether the human bone marrow mesenchymal stem cells （HBMSCs） can repair damaged neural cells induced by okadaic acid （OA）. Methods Neuroblastoma cell line SH-SYSY cells were used to incubate with 20nmol/L okadaic acid for 24h, establishing Mzheimer＇s Disease cell model; Three groups were set up： normal group, okadaic acid -damaged （OA-damaged） group, hBMSCs -treatment group. The cells were injured for 24h with 20nmol/L OA in OA-damaged group, and treated with conditioned medium obtaining hBMSCs for 24h after 24h OA injury in the treatment group. Then CCK-8 was used for detecting cell vitality, immune fluorescence dyed microtubules and microfilaments for determining the dendritic cell length and fluorescence intensity, in addition, Western blotting for analyzing the protein level of phosphorylated tau and total tau proteins. Results Okadaic acid damaged SH-SYSY cells, contributed to shrinkage, collapse, cavitation of the SH-SYSY cell body, dendritic shortening and fracture, and irregular ar ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 |
| PQID | 2001665331 |
| PQPubID | 2047934 |
| PageCount | 6 |
| ParticipantIDs | wanfang_journals_jfjyxzz201705004 proquest_journals_2001665331 chongqing_primary_672204866 |
| PublicationCentury | 2000 |
| PublicationDate | 2017 20170101 |
| PublicationDateYYYYMMDD | 2017-01-01 |
| PublicationDate_xml | – year: 2017 text: 2017 |
| PublicationDecade | 2010 |
| PublicationPlace | Beijing |
| PublicationPlace_xml | – name: Beijing |
| PublicationTitle | Jie fang jun yi xue za zhi |
| PublicationTitleAlternate | Medical Journal of Chinese People＇s Liberation Army |
| PublicationTitle_FL | Medical Journal of Chinese People's Liberation Army |
| PublicationYear | 2017 |
| Publisher | People's Military Medical Press 510515 广州 南方医科大学检验与生物技术学院 100850 北京 军事医学科学院野战输血研究所干细胞与再生医学研究室%100850,北京 军事医学科学院野战输血研究所干细胞与再生医学研究室 |
| Publisher_xml | – name: People's Military Medical Press – name: 100850 北京 军事医学科学院野战输血研究所干细胞与再生医学研究室%100850,北京 军事医学科学院野战输血研究所干细胞与再生医学研究室 – name: 510515 广州 南方医科大学检验与生物技术学院 |
| SSID | ssib001104416 ssib002263991 ssib046619461 ssib051375714 ssj0058562 ssib038074682 ssib052002838 |
| Score | 2.0997095 |
| Snippet | 目的 观察人骨髓间充质干细胞对冈田酸（OA）损伤的神经细胞是否具有修复作用。方法 应用冈田酸损伤神经母细胞瘤细胞系SH-SY5Y细胞,建立阿尔茨海默病体外模型。将细胞分成正... Objective To study whether the human bone marrow mesenchymal stem cells (HBMSCs) can repair damaged neural cells induced by okadaic acid (OA). Methods... R749.16; 目的 观察人骨髓间充质干细胞对冈田酸(OA)损伤的神经细胞是否具有修复作用.方法 应用冈田酸损伤神经母细胞瘤细胞系SH-SY5Y细胞,建立阿尔茨海默病体外模型.将细胞... |
| SourceID | wanfang proquest chongqing |
| SourceType | Aggregation Database Publisher |
| StartPage | 377 |
| SubjectTerms | Acids Alzheimer's disease Bone marrow Stem cells tau蛋白质类 冈田酸 阿尔茨海默病 骨髓间充质干细胞 |
| Title | 骨髓间充质干细胞对冈田酸致神经细胞损伤的修复作用 |
| URI | http://lib.cqvip.com/qk/90166X/201705/672204866.html https://www.proquest.com/docview/2001665331 https://d.wanfangdata.com.cn/periodical/jfjyxzz201705004 |
| Volume | 42 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVAFT databaseName: Open Access Digital Library issn: 0577-7402 databaseCode: KQ8 dateStart: 20110101 customDbUrl: isFulltext: true dateEnd: 99991231 titleUrlDefault: http://grweb.coalliance.org/oadl/oadl.html omitProxy: true ssIdentifier: ssj0058562 providerName: Colorado Alliance of Research Libraries – providerCode: PRVPQU databaseName: East & South Asia Database issn: 0577-7402 databaseCode: BVBZV dateStart: 20110101 customDbUrl: isFulltext: true dateEnd: 99991231 titleUrlDefault: https://search.proquest.com/eastsouthasia omitProxy: false ssIdentifier: ssj0058562 providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central issn: 0577-7402 databaseCode: BENPR dateStart: 20110101 customDbUrl: http://www.proquest.com/pqcentral?accountid=15518 isFulltext: true dateEnd: 99991231 titleUrlDefault: https://www.proquest.com/central omitProxy: true ssIdentifier: ssj0058562 providerName: ProQuest |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3NaxNBFH_UFMVL8ZPGVqnQ69rs7Mzs5lCKSksQGkQq9Bbma1ty2LRaUXPVtF6KBwuCSL1UCAoqkoOliv9M1k3-C-ftbrL14nmWPTzex2_m997vAcyTQHPlVioOM6LiUJ9LG1IV42gT4G7bQEuKs8OrdV57SO-ts_UJqI9mYbCtcpQT00StWwrfyBew94dzC07cpa1tB7dGIbs6WqEh8tUKejGVGDsDkwSVsUoweWe5fv9BARDs7eM0z2bBh63QYw9H9XXKC7UXyvGSX0x-MtfzmV_wjqhZRFLeMcv9FnunK0wtCPIdP-0dmk_TUsDYQjMN61vjM-wry2RDKQo7bLaijW1brv6BumefiigU0capmrdyAaZysDp3O_OuizDR3rwE51ZzOv4y1IafusPPb4Zve3GnM-h14-Pvycnu4MVh_PU43n2VHHwbdn4M9nrJx8Pk5HV29Gf_Q__nUfLuZf_3l_hov__rfXLQvQJrK8trd2tOvprBUUFAHaZCypVSnqssYAqUVAjTqlJasKNdKaikYVA1RPGqtN7gEqGFIT4RJnS1H4beVShFrchMwxySy9rmAekpQZXxBKNeqJjS3HBXKK8MM2PTNLYyBY4G9wkKDnNehtmRsRp58D1uFK5Shpu5AYvTZth8_qzdJqmQkE0R1_7_ixk4j59mry6zUNp59MRctzhkR97Inesv3CrpqQ |
| linkProvider | ProQuest |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3NaxNBFH_UFj8u4ifGVo1Qj2uzs7Ozm0ORqi2pbYJIhN6W-dqWHDatrdTmqml7KT1YEETqpUJQUJEcLLX4z2Td5L9wZneTrRdvPQ87sG_evPd78977PYBx5ArCzULBsCUtGNghTF2pgjSEdPVsW1cwrHuHyxVSeo6fLNgLQ3Dc74XRZZV9mxgbalHn-o18Qtf-EKLAiflgecXQU6N0drU_QoOmoxXEZEwxljZ2zMmNdRXCrU7OPlbnfQ-hmenqo5KRThkwuOtiw-Y-Jpxzy-TK97uccY04iowpvy1MRjHDvluUiJMiUz9mIiqoRA6i0jeF4_uW2vYMjGALF1XsN_JwuvL0WYZHVLBzMq2nsI4CBIMLpcneMcnIZTDRbwpZo6ltWo7tZGlOTZGE4jRn4moU1I8npirM5RhOXKo0HltB17YnarEVuT9Y02VsCUsp1jwSS_VgcUV5x3-Q9dl1Gvg0WDzhYmcuwcUUG-enEmW-DEONpStwrpxm_69Cqfe51fvytveuHTab3XYrPPwRHW12X--H3w7Dze1o73uv-bO71Y4-7UdHu8nSn52PnV8H0fs3nd9fw4OdzvGHaK91DaqncUbXYTioB_IG5HUuWyizwyxOMZcWtbHlc5sLIolJuZWD0YFovOWE8MMjDtL8xoTkYKwvLC-966teppk5uJsKMFut-bWNV40GinmLlEW6-f8t7sD5UrU8783PVuZG4YL-LHnwGYPhtRcv5S0FgdbY7VTR8uCdsmr_BaAGJ8o |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E9%AA%A8%E9%AB%93%E9%97%B4%E5%85%85%E8%B4%A8%E5%B9%B2%E7%BB%86%E8%83%9E%E5%AF%B9%E5%86%88%E7%94%B0%E9%85%B8%E8%87%B4%E7%A5%9E%E7%BB%8F%E7%BB%86%E8%83%9E%E6%8D%9F%E4%BC%A4%E7%9A%84%E4%BF%AE%E5%A4%8D%E4%BD%9C%E7%94%A8&rft.jtitle=%E8%A7%A3%E6%94%BE%E5%86%9B%E5%8C%BB%E5%AD%A6%E6%9D%82%E5%BF%97&rft.au=%E5%88%98%E5%98%89%E5%A9%A7+%E6%9B%B9%E5%AE%81+%E7%BF%9F%E6%99%B6%E7%A3%8A+%E5%BB%96%E5%9C%9F%E7%8E%B2+%E5%B2%B3%E6%96%87+%E8%B4%BE%E9%9B%85%E4%B8%BD+%E8%A3%B4%E9%9B%AA%E6%B6%9B&rft.date=2017&rft.issn=0577-7402&rft.volume=42&rft.issue=5&rft.spage=377&rft.epage=382&rft_id=info:doi/10.11855%2Fj.issn.0577-7402.2017.05.04&rft.externalDocID=672204866 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F90166X%2F90166X.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fjfjyxzz%2Fjfjyxzz.jpg |