骨髓间充质干细胞对冈田酸致神经细胞损伤的修复作用

目的 观察人骨髓间充质干细胞对冈田酸（OA）损伤的神经细胞是否具有修复作用。方法 应用冈田酸损伤神经母细胞瘤细胞系SH-SY5Y细胞,建立阿尔茨海默病体外模型。将细胞分成正常组、损伤组和治疗组,损伤组用20nmol/L冈田酸损伤24h,治疗组在损伤24h后加入骨髓间充质干细胞的条件培养基治疗24h。采用CCK-8法检测各组细胞活力,免疫荧光染色微管微丝测定细胞树突长度和荧光面积,Western blotting检测磷酸化Tau蛋白和总Tau蛋白含量。结果 冈田酸能损伤SH-SY5Y细胞,使其胞体皱缩、塌陷,出现空泡,树突缩短、断裂,微管微丝排列紊乱,而骨髓间充质干细胞条件培养基可使SH-SY5...

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Published inJie fang jun yi xue za zhi Vol. 42; no. 5; pp. 377 - 382
Main Author 刘嘉婧 曹宁 翟晶磊 廖土玲 岳文 贾雅丽 裴雪涛
Format Journal Article
LanguageChinese
Published Beijing People's Military Medical Press 2017
510515 广州 南方医科大学检验与生物技术学院
100850 北京 军事医学科学院野战输血研究所干细胞与再生医学研究室%100850,北京 军事医学科学院野战输血研究所干细胞与再生医学研究室
Subjects
Acids
Alzheimer's disease
Bone marrow
Stem cells
tau蛋白质类
冈田酸
阿尔茨海默病
骨髓间充质干细胞
冈田酸
tau蛋白质类
tau proteins
Alzheimer disease
bone mesenchymal stem cells
骨髓间充质干细胞
阿尔茨海默病
okadaic acid
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ISSN0577-7402
DOI10.11855/j.issn.0577-7402.2017.05.04

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Summary:目的 观察人骨髓间充质干细胞对冈田酸（OA）损伤的神经细胞是否具有修复作用。方法 应用冈田酸损伤神经母细胞瘤细胞系SH-SY5Y细胞,建立阿尔茨海默病体外模型。将细胞分成正常组、损伤组和治疗组,损伤组用20nmol/L冈田酸损伤24h,治疗组在损伤24h后加入骨髓间充质干细胞的条件培养基治疗24h。采用CCK-8法检测各组细胞活力,免疫荧光染色微管微丝测定细胞树突长度和荧光面积,Western blotting检测磷酸化Tau蛋白和总Tau蛋白含量。结果 冈田酸能损伤SH-SY5Y细胞,使其胞体皱缩、塌陷,出现空泡,树突缩短、断裂,微管微丝排列紊乱,而骨髓间充质干细胞条件培养基可使SH-SY5Y细胞胞体变得圆润,树突重新恢复变长,微管、微丝致密规则,荧光变强;并且能有效降低冈田酸诱导的Tau蛋白过度磷酸化水平。结论 骨髓间充质干细胞对冈田酸损伤的神经细胞具有明显的修复作用。
Bibliography:Objective To study whether the human bone marrow mesenchymal stem cells （HBMSCs） can repair damaged neural cells induced by okadaic acid （OA）. Methods Neuroblastoma cell line SH-SYSY cells were used to incubate with 20nmol/L okadaic acid for 24h, establishing Mzheimer＇s Disease cell model; Three groups were set up： normal group, okadaic acid -damaged （OA-damaged） group, hBMSCs -treatment group. The cells were injured for 24h with 20nmol/L OA in OA-damaged group, and treated with conditioned medium obtaining hBMSCs for 24h after 24h OA injury in the treatment group. Then CCK-8 was used for detecting cell vitality, immune fluorescence dyed microtubules and microfilaments for determining the dendritic cell length and fluorescence intensity, in addition, Western blotting for analyzing the protein level of phosphorylated tau and total tau proteins. Results Okadaic acid damaged SH-SYSY cells, contributed to shrinkage, collapse, cavitation of the SH-SYSY cell body, dendritic shortening and fracture, and irregular ar
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ISSN:0577-7402
DOI:10.11855/j.issn.0577-7402.2017.05.04